Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ

Marihan Hegazy, Eran Cohen-Barak, Jennifer L. Koetsier, Nicole A. Najor, Constadina Arvanitis, Eli Sprecher, Kathleen J. Green, Lisa M. Godsel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in situ provides important additional information. The Proximity Ligation Assay® (PLA) offers the opportunity to visualize the subcellular location of such interactions at endogenous protein levels, provided that the probes that recognize the target proteins are within 40 nm. This sensitive technique not only elucidates protein-protein interactions, but also can reveal post-translational protein modifications. The technique is useful even in cases where material is limited, such as when paraffin-embedded clinical specimens are the only available material, as well as after experimental intervention in 2D and 3D model systems. Here we describe the basic protocol for using the commercially available Proximity Ligation Assay™ materials (Sigma-Aldrich, St. Louis, MO), and incorporate details to aid the researcher in successfully performing the experiments.

Original languageEnglish (US)
Pages (from-to)e115
JournalCurrent Protocols in Cell Biology
Volume89
Issue number1
DOIs
StatePublished - Dec 1 2020

Keywords

  • Duolink™
  • in situ tissue staining
  • post-translational modification
  • protein-protein interaction
  • proximity ligation assay®

ASJC Scopus subject areas

  • Cell Biology

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