PSGL-1-mediated adhesion of human hematopoietic progenitors to P- selectin results in suppression of hematopoiesis

Jean Pierre Lévesque, Andrew C.W. Zannettino, Melanie Pudney, Silvana Niutta, David N. Haylock, Karen R. Snapp, Geoffrey S. Kansas, Michael C. Berndt, Paul J. Simmons*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Cellular interactions are critical for the regulation of hematopoiesis. The sialomucin PSGL-1/CD162 mediates the attachment of mature leukocytes to P-selectin. We now show that PSGL-1 also functions as the sole receptor for P-selectin on primitive human CD34+ hematopoietic progenitor cells (HPC). More importantly, ligation of PSGL-1 by immobilized or soluble ligand or anti-PSGL-1 antibody results in a profound suppression of HPC proliferation stimulated by potent combinations of early acting hematopoietic growth factors. These data demonstrate an unanticipated but extremely marked growth- inhibitory effect of P-selectin on hematopoiesis and provide direct evidence that PSGL-1, in addition to its well-documented role as an adhesion molecule on mature leukocytes, is a potent negative regulator of human hematopoietic progenitors.

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalImmunity
Volume11
Issue number3
DOIs
StatePublished - Sep 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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