PSIBLAST_PairwiseStatSig: Reordering PSI-BLAST hits using pairwise statistical significance

Ankit Agrawal*, Xiaoqiu Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


We present an add-on to BLAST and PSI-BLAST programs to reorder their hits using pairwise statistical significance. Using position-specific substitution matrices to estimate pairwise statistical significance has been recently shown to give promising results in terms of retrieval accuracy, which motivates its use to refine PSI-BLAST results, since PSI-BLAST also constructs a position-specific substitution matrix for the query sequence during the search. The obvious advantage of the approach is more accurate estimates of statistical significance because of pairwise statistical significance, along with the advantage of BLAST_PSI-BLAST in terms of speed.

Original languageEnglish (US)
Pages (from-to)1082-1083
Number of pages2
Issue number8
StatePublished - 2009

ASJC Scopus subject areas

  • Computational Mathematics
  • Molecular Biology
  • Biochemistry
  • Statistics and Probability
  • Computer Science Applications
  • Computational Theory and Mathematics


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