Pulse width considerations for multiple-photon excitation laser scanning fluorescence imaging

D. L. Wokosin*, J. G. White, Mike Dymott

*Corresponding author for this work

Research output: Contribution to journalConference article

4 Scopus citations

Abstract

The pulse width considerations for multiple-photon excitation laser scanning imaging were discussed. It was found that with a pre-chirped (compensated) system, when the pulse length was quadrupled, then twice the amount of power was required to attain the previous level of two-photon excited fluorescence and only half the three-photon excitation were produced. The analysis suggested the use of variation in pulse widths for live cell studies.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3260
DOIs
StatePublished - Dec 1 1998
EventOptical Investigations of Cells In Vitro and In Vivo - San Jose, CA, United States
Duration: Jan 25 1998Jan 28 1998

Keywords

  • Laser scanning fluorescence microscopy
  • Phototoxicity
  • Three-photon excitation
  • Two-photon excitation
  • Ultrafast pulsed lasers
  • UV irradiation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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