Purification and characterization of two cellulase free xylanases from an alkaliphilic Bacillus firmus

Min Jen Tseng*, Mee-Ngan Frances Yap, Khanok Ratanakhanokchai, Khin Lay Kyu, Shui Tein Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Two xylanases from Bacillus firmus were purified to homogeneity by gel filtration and ion-exchange chromatography. These enzymes have molecular weights of 45 kDa and 23 kDa, respectively, and both show enzymatic activity over the pH range of 5.0-11.0 at 37°C. These enzymes hydrolyzed xylan from birchwood to release mainly the products of xylose, xylotriose and xylohexose, thus indicating that the xylanases act preferentially toward the internal glycosidic bonds of xylo-oligosaccharides. However, the two xylanases show different modes of action, and a combination of both is likely to lead to concerted degradation of xylan down to the mono- and disaccharides.

Original languageEnglish (US)
Pages (from-to)590-595
Number of pages6
JournalEnzyme and Microbial Technology
Volume30
Issue number5
DOIs
StatePublished - May 2 2002

Keywords

  • Bacillus firmus
  • Enzyme purification
  • Xylanase action
  • Xylanases

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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