Purification and chemical modifications of hemoglobin in developing hemoglobin based oxygen carriers

Chad R. Haney, Paul W. Buehler, Anil Gulati*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    52 Scopus citations

    Abstract

    The efficacy of blood substitutes, as a whole, has been readily demonstrated, in animals as well as clinical studies. It is well known that stroma free hemoglobin (SF-Hb) is very toxic, due to effects on renal and coagulation functions and vascular tone. Several modifications have been made to the hemoglobin tetramer in an attempt to eliminate its toxicity. Conjugation, cross-linking, polymerization, and recombinant technology have all been used to reduce toxicity, while aiming to optimize the therapeutic value of hemoglobin based blood substitutes. The remaining issue seems to be the hypertensive response seen in many hemoglobin solutions. The cause of the hypertensive response, and hence what chemical modifications are suitable to alleviate it are still under debate.

    Original languageEnglish (US)
    Pages (from-to)153-169
    Number of pages17
    JournalAdvanced Drug Delivery Reviews
    Volume40
    Issue number3
    DOIs
    StatePublished - Feb 28 2000

    Keywords

    • Blood substitutes
    • Cross-linking
    • Hypertensive drug effects
    • Polymerization

    ASJC Scopus subject areas

    • Pharmaceutical Science

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