Purification of a membrane protein distributed in a topographic gradient in chicken retina

J. R. Moskal, D. Trisler, M. D. Schneider, M. Nirenberg

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Antigenic molecules termed TOP, which are distributed in a dorsal > ventral concentration gradient in chicken retina, are expressed early in development (by 48 hr after fertilization) in the optic cup of chicken embryos and continue to be expressed in retina thereafter. 35S-labeled-TOP-antibody complexes were purified by protein A-Sepharose column chromatography and subjected to NaDodDO4/polyacrylamide gel electrophoresis and autoradiography. TOP also was purified from dorsal retina by anti-TOP IgG-Affi-Gel 10 affinity dolumn chromatography. In both cases, one major band of protein at M(r) ≃ 47,000 was obtained. A protein of M(r) ≃ 47,000 also was purified from chicken embryo brain. Cultured cells dissociated from 8-day chicken embryo retinas accumulated the amount of TOP expected of cells in the intact retina, depending on the position of the cells in the retina. TOP accumulations by cells dissociated from dorsal or ventral retina, mixed in different proportions, and cocultured were additive. These results show that TOP is a protein, that the gradient of TOP is established early in development, and that perpetuation of the gradient does not depend on the continuous presence of an extracellular gradient of diffusable molecules or on maintenance of interactions between cells.

Original languageEnglish (US)
Pages (from-to)4730-4733
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number13
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Purification of a membrane protein distributed in a topographic gradient in chicken retina'. Together they form a unique fingerprint.

Cite this