Purification of prominin-1+ stem cells from postnatal mouse cerebellum

Chandrakanth Reddy Edamakanti, Puneet Opal*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Most cerebellar neurons arise from two embryonic stem niches: a rhombic lip niche, which generates all the cerebellar excitatory glutamatergic neurons, and a ventricular zone niche, which generates the inhibitory GABAergic Purkinje cells, which are neurons that constitute the deep cerebellar nuclei and Bergman glia. Recently, a third stem cell niche has been described that arises as a secondary germinal zone from the ventricular zone niche. The cells of this niche are defined by the cell surface marker prominin-1 and are localized to the developing white matter of the postnatal cerebellum. This niche accounts for the late born molecular layer GABAergic interneurons along with postnatally generated cerebellar astrocytes. In addition to their developmental role, this niche is gaining translational importance in regards to its involvement in neurodegeneration and tumorigenesis. The biology of these cells has been difficult to decipher because of a lack of efficient techniques for their purification. Demonstrated here are efficient methods to purify, culture, and differentiate these postnatal cerebellar stem cells.

Original languageEnglish (US)
Article numbere60554
JournalJournal of Visualized Experiments
Volume2020
Issue number158
DOIs
StatePublished - Apr 2020

Keywords

  • Astrocytes
  • Cerebellum
  • Interneurons
  • Issue 158
  • Neural stem cells
  • Neuroscience
  • Postnatal
  • Prominin-1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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