Purifying protein complexes for mass spectrometry: Applications to protein translation

Andrew J. Link*, Tracey C. Fleischer, Connie M. Weaver, Robert V Gerbasi, Jennifer L. Jennings

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Proteins control and mediate most of the biological activities in the cell. In most cases, proteins either interact with regulatory proteins or function in large molecular assemblies to carryout biological processes. Understanding the functions of individual proteins requires the identification of these interacting proteins. With its speed and sensitivity, mass spectrometry has become the dominant method for identifying components of protein complexes. This article reviews and discusses various approaches to purify protein complexes and analyze the proteins using mass spectrometry. As examples, methods to isolate and analyze protein complexes responsible for the translation of messenger RNAs into polypeptides are described.

Original languageEnglish (US)
Pages (from-to)274-290
Number of pages17
JournalMethods
Volume35
Issue number3 SPEC.ISS.
DOIs
StatePublished - Mar 2005

Keywords

  • Affinity chromatography
  • Database searching
  • Epitope tagging
  • Interactions
  • Mass spectrometry
  • Protein complexes
  • Purifications
  • Translation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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