@article{aa02af39a3b34330ba4ad058ddafbe17,
title = "Purkinje cells of adult rat cerebellum express nerve growth factor receptor immunoreactivity: light microscopic observations",
abstract = "Adult rat cerebellum was examined for the presence of nerve growth factor receptor (NGFr) immunoreactivity (IR) using the monoclonal antibody 192-IgG. In normal animals, light NGFr-IR was observed consistently in Purkinje cells and molecular layer throughout the flocculonodular lobe. However, animals treated with intracerebroventricular colchicine 7-72 h prior to analysis displayed a graded increase of IR in Purkinje cells of all regions with progressively longer survival times. These findings demonstrate the usefulness of colchicine in revealing accumulation of NGFr in adult CNS neurons where previously not visualized and suggest responsiveness of a non-cholinergic neuronal population to nerve growth factor (NGF).",
keywords = "Cerebellum, Colchicine, Flocculonodular lobe, Immunoreactivity, Nerve growth factor, Nerve growth factor receptor, Purkinje cell",
author = "Pioro, {Erik P.} and {Claudio Cuello}, A.",
note = "Funding Information: Support for this study was provided by the Medical Research Council (Canada) and Office of the Dean, Faculty of Medicine, McGill University. The authors thank Dr. E.M. Johnson, Jr. for his gift of hybridoma 1 Chandler, C.E., Parsons, L.M., Hosang, M. and Shooter, E.M., A monoclonal antibody modulates the interaction of nerve growth factor with PC12 cells, J. Biol. Chem., 259 (1984) 6882-6889. 2 Cuello, A.C., Del Fiacco-Lampis, M, and Paxinos, G., Combined immunocytochemistry with stereotaxic lesions. In A.C. Cuello (Ed.), lmmunocytochemistry, 1BRO Hand-book Series: Methods in the Neurosciences, Vol. 3, Wiley, Chichester, 1983, pp. 477-496. 3 Dasheiff, R.M. and Ramirez, L.F., The effects of colchi-cine in mammalian brain from rodents to rhesus monkeys, Brain Res. Rev., !0 (1985) 47-67. 4 Eckenstein, F.E., Distribution of NGF-receptor-like im-munoreactivity in the postnatal rat brain, Soc. Neurosci. Abstr., 13 (1987) 518. 5 Fischer, W., Wictorin, K., Bj6rklund, A., Williams, L.R., Varon, S. and Gage, F.H., Amelioration of cholinergic neuron atrophy and spatial memory impairment in aged rats by nerve growth factor, Nature (Lond.), 329 (1987) 65-68. 6 Gnahn, H., Hefti, F., Heumann, R., Schwab, M.E. and Thoenen, H., NGF-mediated increase of choline acetyl-transferase (CHAT) in the neonatal rat forebrain: evidence for a physiological role of NGF in the brain?, Dev. Brain Res., 9 (1983) 45-52. 7 Gomez-Pinilla, F., Cotman, C.W. and Nieto-Sampedro, M., NGF receptor immunoreactivity in rat brain: topo-graphical distribution and response to entorhinal ablation, Neurosci. Lett., 82 (1987) 260-266. 8 Gould, E. and Butcher, L.L., Transient expression of cho-line acetyltransferase-like immunoreactivity in Purkinje cells of the developing rat cerebellum, Dev. Brain Res., 34 (1987) 303-306. 9 Green, S.H. and Greene, L.A., A single Mr = 103,000 125I-fl nerve growth factor-affinity-labeled species repre-sents both the low and high affinity forms of the nerve growth factor receptor, J. Biol. Chem., 261 (1986) 15316-15326. 10 Hefti, F., Dravid, A. and Hartikka, J., Chronic intraven-tricular injections of nerve growth factor elevate hippocam-pal choline acetyltransferase activity in adult rats with par-tial septo-hippocampal lesions, Brain Research, 293 (1984) 305-311. 11 Hefti, F., Hartikka, J., Eckenstein, F., Gnahn, H., Heu-mann, R. and Schwab, M., Nerve growth factor increases choline acetyltransferase but not survival or fiber out-growth of cultured fetal septal cholinergic neurons, Neuro-science, 14 (1985) 55-68. 12 Large, T.H., Bodary, S.C., Clegg, D.O., Weskamp, G., Otten, U. and Reichardt, L.F., Nerve growth factor gene expression in the developing rat brain, Science, 234 (1986) 352-355. 13 Levi-Montalcini, R. and Aloe, L., Differentiating effects of murine nerve growth factor in the peripheral and central nervous system of Xenopus laevis tadpoles, Proc. Natl.",
year = "1988",
month = jul,
day = "5",
doi = "10.1016/0006-8993(88)90131-X",
language = "English (US)",
volume = "455",
pages = "182--186",
journal = "Brain research",
issn = "0006-8993",
publisher = "Elsevier B.V.",
number = "1",
}