QRFP and its receptors regulate locomotor activity and sleep in zebrafish

Audrey Chen, Cindy N. Chiu, Eric A. Mosser, Sohini Kahn, Rory Spence, David A. Prober*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


The hypothalamus plays an important role in regulating sleep, but few hypothalamic sleep-promoting signaling pathways have been identified. Here we demonstrate a role for the neuropeptide QRFP (also known as P518 and 26RFa) and its receptors in regulating sleep in zebrafish, a diurnal vertebrate. Weshow that QRFP is expressed in ~ 10 hypothalamic neurons in zebrafish larvae, which project to the hypothalamus, hindbrain, and spinal cord, including regions that express the two zebrafish QRFP receptor paralogs. We find that the overexpression of QRFP inhibits locomotor activity during the day, whereas mutation of qrfp or its receptors results in increased locomotor activity and decreased sleep during the day. Despite the restriction of these phenotypes to the day, the circadian clock does not regulate qrfp expression, and entrained circadian rhythms are not required for QRFP-induced rest. Instead, we find that QRFP overexpression decreases locomotor activity largely in a light-specific manner. Our results suggest that QRFP signaling plays an important role in promoting sleep and may underlie some aspects of hypothalamic sleep control.

Original languageEnglish (US)
Pages (from-to)1823-1840
Number of pages18
JournalJournal of Neuroscience
Issue number6
StatePublished - Feb 10 2016


  • 26RFa
  • Gpr103
  • P518
  • QRFP
  • Sleep
  • Zebrafish

ASJC Scopus subject areas

  • Neuroscience(all)

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