Quantification of cardiac fiber orientation using optical coherence tomography

Christine P. Fleming; Crystal M. Ripplinger; Bryan Webb; Igor R. Efimov; Andrew M. Rollins

doi:10.1117/1.2937470

Quantification of cardiac fiber orientation using optical coherence tomography

Christine P. Fleming^*, Crystal M. Ripplinger, Bryan Webb, Igor R. Efimov, Andrew M. Rollins

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.

Original language	English (US)
Article number	030505
Journal	Journal of Biomedical Optics
Volume	13
Issue number	3
DOIs	https://doi.org/10.1117/1.2937470
State	Published - 2008

Funding

The project described was supported by Grant Nos. 1RO1HL08304, R01-HL-67322, R01-HL-074283, F31 HL085939, C06RR1246–01 (NIH) and 0515562Z (American Heart Association).

Keywords

biomedical optics
cardiac
image processing
optical coherence tomography

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.2937470

Cite this

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title = "Quantification of cardiac fiber orientation using optical coherence tomography",

abstract = "Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.",

keywords = "biomedical optics, cardiac, image processing, optical coherence tomography",

author = "Fleming, {Christine P.} and Ripplinger, {Crystal M.} and Bryan Webb and Efimov, {Igor R.} and Rollins, {Andrew M.}",

note = "Funding Information: The project described was supported by Grant Nos. 1RO1HL08304, R01-HL-67322, R01-HL-074283, F31 HL085939, C06RR1246–01 (NIH) and 0515562Z (American Heart Association).",

year = "2008",

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language = "English (US)",

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AU - Fleming, Christine P.

AU - Ripplinger, Crystal M.

AU - Webb, Bryan

AU - Efimov, Igor R.

AU - Rollins, Andrew M.

N1 - Funding Information: The project described was supported by Grant Nos. 1RO1HL08304, R01-HL-67322, R01-HL-074283, F31 HL085939, C06RR1246–01 (NIH) and 0515562Z (American Heart Association).

PY - 2008

Y1 - 2008

N2 - Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.

AB - Heterogeneity in cardiac tissue microstructure is a potential mechanism for the generation and maintenance of arrhythmias. Abnormal changes in fiber orientation increase the likelihood of arrhythmia. We present optical coherence tomography (OCT) as a method to image myofibers in excised intact heart preparations. Three-dimensional (3-D) image sets were gathered from the rabbit right ventricular free wall (RVFW) using a microscope-integrated OCT system. An automated algorithm for fiber orientation quantification in the plane parallel to the wall surface was developed. The algorithm was validated by comparison with manual measurements. Quantifying fiber orientation in the plane parallel to the wall surface from OCT images can be used to help understand the conduction system of the specific sample being imaged.

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KW - image processing

KW - optical coherence tomography

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Quantification of cardiac fiber orientation using optical coherence tomography

Abstract

Funding

Keywords

ASJC Scopus subject areas

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