Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology

Yuqi Zhao; Min Yu; Johann W. Miller; Mingzhong Chen; Eric G. Bremer; William Kabat; Ram Yogev

doi:10.1128/JCM.40.2.675-678.2002

Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology

Yuqi Zhao^*, Min Yu, Johann W. Miller, Mingzhong Chen, Eric G. Bremer, William Kabat, Ram Yogev

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.

Original language	English (US)
Pages (from-to)	675-678
Number of pages	4
Journal	Journal of clinical microbiology
Volume	40
Issue number	2
DOIs	https://doi.org/10.1128/JCM.40.2.675-678.2002
State	Published - 2002

ASJC Scopus subject areas

Microbiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.40.2.675-678.2002

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abstract = "A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.",

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AU - Zhao, Yuqi

AU - Yu, Min

AU - Miller, Johann W.

AU - Chen, Mingzhong

AU - Bremer, Eric G.

AU - Kabat, William

AU - Yogev, Ram

PY - 2002

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Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology

Abstract

ASJC Scopus subject areas

UN SDGs

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