Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology

Yuqi Zhao*, Min Yu, Johann W. Miller, Mingzhong Chen, Eric G. Bremer, William Kabat, Ram Yogev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.

Original languageEnglish (US)
Pages (from-to)675-678
Number of pages4
JournalJournal of clinical microbiology
Volume40
Issue number2
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Microbiology (medical)

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