Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

T. Yeghiazarian*, Y. Zhao, S. E. Read, W. Kabat, X. Li, S. J. Hamren, P. J. Sheridan, J. C. Wilber, D. N. Chernoff, R. Yogev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have developed small-volume (50 or 250 μl)-format branched DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

Original languageEnglish (US)
Pages (from-to)2096-2098
Number of pages3
JournalJournal of clinical microbiology
Volume36
Issue number7
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Microbiology (medical)

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