Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

T. Yeghiazarian; Y. Zhao; S. E. Read; W. Kabat; X. Li; S. J. Hamren; P. J. Sheridan; J. C. Wilber; D. N. Chernoff; R. Yogev

doi:10.1128/jcm.36.7.2096-2098.1998

Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

T. Yeghiazarian^*, Y. Zhao, S. E. Read, W. Kabat, X. Li, S. J. Hamren, P. J. Sheridan, J. C. Wilber, D. N. Chernoff, R. Yogev

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We have developed small-volume (50 or 250 μl)-format branched DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

Original language	English (US)
Pages (from-to)	2096-2098
Number of pages	3
Journal	Journal of clinical microbiology
Volume	36
Issue number	7
DOIs	https://doi.org/10.1128/jcm.36.7.2096-2098.1998
State	Published - 1998

ASJC Scopus subject areas

Microbiology (medical)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/jcm.36.7.2096-2098.1998

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title = "Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays",

abstract = "We have developed small-volume (50 or 250 μl)-format branched DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.",

author = "T. Yeghiazarian and Y. Zhao and Read, {S. E.} and W. Kabat and X. Li and Hamren, {S. J.} and Sheridan, {P. J.} and Wilber, {J. C.} and Chernoff, {D. N.} and R. Yogev",

year = "1998",

doi = "10.1128/jcm.36.7.2096-2098.1998",

language = "English (US)",

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T1 - Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

AU - Yeghiazarian, T.

AU - Zhao, Y.

AU - Read, S. E.

AU - Kabat, W.

AU - Li, X.

AU - Hamren, S. J.

AU - Sheridan, P. J.

AU - Wilber, J. C.

AU - Chernoff, D. N.

AU - Yogev, R.

PY - 1998

Y1 - 1998

N2 - We have developed small-volume (50 or 250 μl)-format branched DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

AB - We have developed small-volume (50 or 250 μl)-format branched DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

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UR - http://www.scopus.com/inward/citedby.url?scp=0031837572&partnerID=8YFLogxK

U2 - 10.1128/jcm.36.7.2096-2098.1998

DO - 10.1128/jcm.36.7.2096-2098.1998

M3 - Article

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AN - SCOPUS:0031837572

SN - 0095-1137

VL - 36

SP - 2096

EP - 2098

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 7

ER -

Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

Abstract

ASJC Scopus subject areas

UN SDGs

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