Quantification of human immunodeficiency virus type 1 tat mrna as a marker for assessing the efficacy of antiretroviral therapy

Manohar R. Furtado*, Robert Murphy, Steven M. Wolinsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

To evaluate the use of human immunodeficiency virus type 1 (HIV-1) tat mRNA quantification as a marker for antiretroviral therapy, 10 zidovudine-naive, p24 antibody-positive subjects (Centers for Disease Control classes III and IV) were studied at the start ofzidovudine treatment. HIV-1 proviral DNA content and tat mRNA levels were monitored for 20 weeks from the initiation of therapy. Levels of tat mRNA were quantified using an engineered tat cRNA internal control under conditions of linear amplification. Proviral DNA levels increased in 2 patients and decreased in 8 during 20 weeks of therapy. In each case, tat mRNA levels exhibited similar but much more pronounced changes. Results indicate that quantitative measurement of tat mRNA levels is extremely useful for monitoring antiretroviral therapy.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalJournal of Infectious Diseases
Volume167
Issue number1
DOIs
StatePublished - Jan 1993

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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