Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Faye B. Vicente, Gina Vespa, Alan Miller, Shannon Haymond

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Iohexol is a nonradioactive contrast medium, and its clearance from serum or urine is used to measure glomerular filtration rate (GFR). GFR is the most useful indicator of kidney function and progression of kidney disease. GFR determination using iohexol clearance is increasingly being applied in clinical practice, given its advantages over and correlation with inulin. We describe a high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method for iohexol clearance, requiring only 50 μL of serum. The sample preparation involves protein precipitation with LC/MS-grade methanol, containing ioversol as the internal standard. Samples are centrifuged and supernatant is dried under nitrogen gas at room temperature. Samples are reconstituted with mobile phase (ammonium acetate-formic acid- water). Iohexol is separated using an HPLC gradient method on a C-8 analytical column. MS/MS detection is in the multiple-reaction monitoring (MRM) mode and the transitions monitored are m/z 822.0 to m/z 804.0 and m/z 807.0 to m/z 588.0 for iohexol and ioversol, respectively.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages185-193
Number of pages9
DOIs
StatePublished - May 1 2016

Publication series

NameMethods in Molecular Biology
Volume1383
ISSN (Print)1064-3745

Fingerprint

Iohexol
Tandem Mass Spectrometry
ioversol
High Pressure Liquid Chromatography
Glomerular Filtration Rate
Serum
formic acid
Inulin
Kidney Diseases
Contrast Media
Methanol
Nitrogen
Gases
Urine
Kidney
Temperature
Water
Proteins

Keywords

  • Glomerular filtration rate
  • Iohexol
  • Liquid chromatography
  • Mass spectrometry
  • Quantification
  • Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vicente, F. B., Vespa, G., Miller, A., & Haymond, S. (2016). Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). In Methods in Molecular Biology (pp. 185-193). (Methods in Molecular Biology; Vol. 1383). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3252-8_20
Vicente, Faye B. ; Vespa, Gina ; Miller, Alan ; Haymond, Shannon. / Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods in Molecular Biology. Humana Press Inc, 2016. pp. 185-193 (Methods in Molecular Biology).
@inbook{a72518d1d1af4479890a123d5b451367,
title = "Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)",
abstract = "Iohexol is a nonradioactive contrast medium, and its clearance from serum or urine is used to measure glomerular filtration rate (GFR). GFR is the most useful indicator of kidney function and progression of kidney disease. GFR determination using iohexol clearance is increasingly being applied in clinical practice, given its advantages over and correlation with inulin. We describe a high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method for iohexol clearance, requiring only 50 μL of serum. The sample preparation involves protein precipitation with LC/MS-grade methanol, containing ioversol as the internal standard. Samples are centrifuged and supernatant is dried under nitrogen gas at room temperature. Samples are reconstituted with mobile phase (ammonium acetate-formic acid- water). Iohexol is separated using an HPLC gradient method on a C-8 analytical column. MS/MS detection is in the multiple-reaction monitoring (MRM) mode and the transitions monitored are m/z 822.0 to m/z 804.0 and m/z 807.0 to m/z 588.0 for iohexol and ioversol, respectively.",
keywords = "Glomerular filtration rate, Iohexol, Liquid chromatography, Mass spectrometry, Quantification, Serum",
author = "Vicente, {Faye B.} and Gina Vespa and Alan Miller and Shannon Haymond",
year = "2016",
month = "5",
day = "1",
doi = "10.1007/978-1-4939-3252-8_20",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "185--193",
booktitle = "Methods in Molecular Biology",

}

Vicente, FB, Vespa, G, Miller, A & Haymond, S 2016, Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1383, Humana Press Inc, pp. 185-193. https://doi.org/10.1007/978-1-4939-3252-8_20

Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). / Vicente, Faye B.; Vespa, Gina; Miller, Alan; Haymond, Shannon.

Methods in Molecular Biology. Humana Press Inc, 2016. p. 185-193 (Methods in Molecular Biology; Vol. 1383).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)

AU - Vicente, Faye B.

AU - Vespa, Gina

AU - Miller, Alan

AU - Haymond, Shannon

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Iohexol is a nonradioactive contrast medium, and its clearance from serum or urine is used to measure glomerular filtration rate (GFR). GFR is the most useful indicator of kidney function and progression of kidney disease. GFR determination using iohexol clearance is increasingly being applied in clinical practice, given its advantages over and correlation with inulin. We describe a high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method for iohexol clearance, requiring only 50 μL of serum. The sample preparation involves protein precipitation with LC/MS-grade methanol, containing ioversol as the internal standard. Samples are centrifuged and supernatant is dried under nitrogen gas at room temperature. Samples are reconstituted with mobile phase (ammonium acetate-formic acid- water). Iohexol is separated using an HPLC gradient method on a C-8 analytical column. MS/MS detection is in the multiple-reaction monitoring (MRM) mode and the transitions monitored are m/z 822.0 to m/z 804.0 and m/z 807.0 to m/z 588.0 for iohexol and ioversol, respectively.

AB - Iohexol is a nonradioactive contrast medium, and its clearance from serum or urine is used to measure glomerular filtration rate (GFR). GFR is the most useful indicator of kidney function and progression of kidney disease. GFR determination using iohexol clearance is increasingly being applied in clinical practice, given its advantages over and correlation with inulin. We describe a high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method for iohexol clearance, requiring only 50 μL of serum. The sample preparation involves protein precipitation with LC/MS-grade methanol, containing ioversol as the internal standard. Samples are centrifuged and supernatant is dried under nitrogen gas at room temperature. Samples are reconstituted with mobile phase (ammonium acetate-formic acid- water). Iohexol is separated using an HPLC gradient method on a C-8 analytical column. MS/MS detection is in the multiple-reaction monitoring (MRM) mode and the transitions monitored are m/z 822.0 to m/z 804.0 and m/z 807.0 to m/z 588.0 for iohexol and ioversol, respectively.

KW - Glomerular filtration rate

KW - Iohexol

KW - Liquid chromatography

KW - Mass spectrometry

KW - Quantification

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=84965134157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84965134157&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3252-8_20

DO - 10.1007/978-1-4939-3252-8_20

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 185

EP - 193

BT - Methods in Molecular Biology

PB - Humana Press Inc

ER -

Vicente FB, Vespa G, Miller A, Haymond S. Quantification of iohexol in serum by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). In Methods in Molecular Biology. Humana Press Inc. 2016. p. 185-193. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3252-8_20