Quantification of sarcomere length distribution in whole muscle frozen sections

Shawn M. O'Connor; Elton J. Cheng; Kevin W. Young; Samuel R. Ward; Richard L. Lieber

doi:10.1242/jeb.132084

Quantification of sarcomere length distribution in whole muscle frozen sections

Shawn M. O'Connor, Elton J. Cheng, Kevin W. Young, Samuel R. Ward, Richard L. Lieber^*

^*Corresponding author for this work

Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Laser diffraction (LD) is a valuable tool for measuring sarcomere length (L_s), a major determinant of muscle function. However, this method relies on few measurements per sample that are often extrapolated to whole muscle properties. Currently it is not possible to measure L_s throughout an entire muscle and determine how L_s varies at this scale. To address this issue, we developed an actuated LD scanner for sampling large numbers of sarcomeres in thick whole muscle longitudinal sections. Sections of high optical quality and fixation were produced from tibialis anterior and extensor digitorum longus muscles of Sprague-Dawley rats (N=6). Scans produced twodimensional L_s maps, capturing >85% of the muscle area per section. Individual L_s measures generated by automatic LD and bright-field microscopy showed excellent agreement over a large L_s range (ICC>0.93). Two-dimensional maps also revealed prominent regional L_s variations across muscles.

Original language	English (US)
Pages (from-to)	1432-1436
Number of pages	5
Journal	Journal of Experimental Biology
Volume	219
Issue number	10
DOIs	https://doi.org/10.1242/jeb.132084
State	Published - May 15 2016

Keywords

Laser diffraction
Muscle architecture
Sarcomere length

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Physiology
Aquatic Science
Animal Science and Zoology
Molecular Biology
Insect Science

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title = "Quantification of sarcomere length distribution in whole muscle frozen sections",

abstract = "Laser diffraction (LD) is a valuable tool for measuring sarcomere length (Ls), a major determinant of muscle function. However, this method relies on few measurements per sample that are often extrapolated to whole muscle properties. Currently it is not possible to measure Ls throughout an entire muscle and determine how Ls varies at this scale. To address this issue, we developed an actuated LD scanner for sampling large numbers of sarcomeres in thick whole muscle longitudinal sections. Sections of high optical quality and fixation were produced from tibialis anterior and extensor digitorum longus muscles of Sprague-Dawley rats (N=6). Scans produced twodimensional Ls maps, capturing >85% of the muscle area per section. Individual Ls measures generated by automatic LD and bright-field microscopy showed excellent agreement over a large Ls range (ICC>0.93). Two-dimensional maps also revealed prominent regional Ls variations across muscles.",

keywords = "Laser diffraction, Muscle architecture, Sarcomere length",

author = "O'Connor, {Shawn M.} and Cheng, {Elton J.} and Young, {Kevin W.} and Ward, {Samuel R.} and Lieber, {Richard L.}",

note = "Funding Information: This work was supported by the National Institutes of Health [P30 AR058878, R24 HD050837]; and the US Department of Veterans Affairs. Deposited in PMC for release after 12 months.",

year = "2016",

month = may,

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doi = "10.1242/jeb.132084",

language = "English (US)",

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AU - O'Connor, Shawn M.

AU - Cheng, Elton J.

AU - Young, Kevin W.

AU - Ward, Samuel R.

AU - Lieber, Richard L.

N1 - Funding Information: This work was supported by the National Institutes of Health [P30 AR058878, R24 HD050837]; and the US Department of Veterans Affairs. Deposited in PMC for release after 12 months.

PY - 2016/5/15

Y1 - 2016/5/15

N2 - Laser diffraction (LD) is a valuable tool for measuring sarcomere length (Ls), a major determinant of muscle function. However, this method relies on few measurements per sample that are often extrapolated to whole muscle properties. Currently it is not possible to measure Ls throughout an entire muscle and determine how Ls varies at this scale. To address this issue, we developed an actuated LD scanner for sampling large numbers of sarcomeres in thick whole muscle longitudinal sections. Sections of high optical quality and fixation were produced from tibialis anterior and extensor digitorum longus muscles of Sprague-Dawley rats (N=6). Scans produced twodimensional Ls maps, capturing >85% of the muscle area per section. Individual Ls measures generated by automatic LD and bright-field microscopy showed excellent agreement over a large Ls range (ICC>0.93). Two-dimensional maps also revealed prominent regional Ls variations across muscles.

AB - Laser diffraction (LD) is a valuable tool for measuring sarcomere length (Ls), a major determinant of muscle function. However, this method relies on few measurements per sample that are often extrapolated to whole muscle properties. Currently it is not possible to measure Ls throughout an entire muscle and determine how Ls varies at this scale. To address this issue, we developed an actuated LD scanner for sampling large numbers of sarcomeres in thick whole muscle longitudinal sections. Sections of high optical quality and fixation were produced from tibialis anterior and extensor digitorum longus muscles of Sprague-Dawley rats (N=6). Scans produced twodimensional Ls maps, capturing >85% of the muscle area per section. Individual Ls measures generated by automatic LD and bright-field microscopy showed excellent agreement over a large Ls range (ICC>0.93). Two-dimensional maps also revealed prominent regional Ls variations across muscles.

KW - Laser diffraction

KW - Muscle architecture

KW - Sarcomere length

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