Abstract
Neurodevelopmental disorders are characterized by complex phenotypes that often result from concomitant dysregulation of cell proliferation, differentiation, or other crucial developmental processes. Here, we present a protocol to quantify differentiation of progenitor populations during early stages of neurogenesis in induced pluripotent stem cell (iPSC)-derived cerebral organoids. We describe steps for organoid differentiation and maturation, sample preparation, immunofluorescence, and imaging and analysis using epifluorescence microscopy. This protocol can be used to compare cerebral organoids from control and patient-derived iPSCs. For complete details on the use and execution of this protocol, please refer to Rakotomamonjy et al. (2023).1
Original language | English (US) |
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Article number | 102904 |
Journal | STAR Protocols |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - Mar 15 2024 |
Funding
We thank Dr. Talia Lerner for sharing imaging equipment. The graphical abstract was made using BioRender. This study was supported by the NIH ( T32 GM008061 to A.L.S., R00NS089943 and R01NS123163 to A.G.-G., and diversity supplement for R01NS123163 to M.F.-S.).
Keywords
- Cell Differentiation
- Microscopy
- Neuroscience
- Organoids
- Stem Cells
ASJC Scopus subject areas
- General Immunology and Microbiology
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience