Quantitation of acute necrosis after experimental myocardial infarction

Xin Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, Edward B. Thorp

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations


Myocardial infarction (MI) is death and necrosis of myocardial tissue secondary to ischemia. MI is associated with adverse cardiac remodeling, progressive heart chamber dilation, ventricular wall thinning, and loss of cardiac function. Myocardial necrosis can be experimentally induced in rodents to simulate human MI by surgical occlusion of coronary arteries. When induced in knockout or transgenic mice, this model is useful for the identification of molecular modulators of cell death, cardiac remodeling, and preclinical therapeutic potential. Herein we outline in tandem, methods for microsurgical ligation of the left anterior descending artery followed by quantitation of myocardial necrosis. Necrosis is quantified after staining the heart with triphenyltetrazolium chloride.

Original languageEnglish (US)
Title of host publicationNecrosis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages19
ISBN (Print)9781627033824
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Area at risk (AAR)
  • Infarct size
  • Myocardial infarction
  • Necrosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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