Quantitation of human immunodeficiency virus type 1 DNA and RNA by a novel internally controlled PCR assay

P. Gupta*, M. Ding, M. Cottrill, C. Rinaldo, L. Kingsley, S. Wolinsky, J. Mellors

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A novel internally controlled PCR (ICPCR) assay was developed to accurately quantitate human immunodeficiency virus type 1 (HIV-1) DNA and RNA in peripheral blood mononuclear cells and plasma. The ICPCR assay was sensitive and reproducible within a linear range of amplification of 100 to 103 copies for HIV-1 DNA and 101 to 104 copies for HIV-1 RNA. The assay detected HIV-1 RNA in plasma and peripheral blood mononuclear cells from all HIV-1 subjects regardless of disease stage. ICPCR was compared with a branched-DNA signal amplification assay for subjects beginning antiretroviral therapy. The reductions in plasma HIV-1 RNA in response to therapy were comparable with the two assays. The ICPCR assay should be useful in monitoring HIV-1 RNA levels both in natural history studies and in clinical trials of antiretroviral agents.

Original languageEnglish (US)
Pages (from-to)1670-1673
Number of pages4
JournalJournal of clinical microbiology
Volume33
Issue number6
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Microbiology (medical)

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