Quantitation of human immunoglobulin G and albumin in electroimmunodiffusion gels containing ionic and nonionic detergents

Paul Shapshak*, Wallace W. Tourtellotte, Susan Staugaitis, Tina Cowan, Timothy Ingram, Marvin L. Weil, David Bliss, Warren G. Tourtellotte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Quantitation of human immunoglobulin G (IgG) and albumin by agarose electroimmuno-diffusion is influenced by the incorporation of ionic and nonionic detergents in the gel. The highest concentrations of each detergent at which human IgG and albumin determinations could be performed without perturbing the quantitations were 4% Triton X-100, 4% Tween 80, 1% NP-40, 0.5% sodium deoxycholate (SDOC), 0.5% Zwittergent, and 0.1% sodium dodecyl sulfate (SDS), and mixtures of Triton X-100, SDOC, and SDS. These detergent combinations all resulted in greater perturbations of albumin quantitation than of IgG. Immunoprecipitation of human IgG was quantitated in the absence and presence of Triton X-100, Zwittergent, and SDS. SDS was shown to cause nonspecific precipitation, whereas below 1% Triton X-100 or 0.5% Zwittergent no effects upon the immunoprecipitations were observed.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalAnalytical Biochemistry
Volume132
Issue number2
DOIs
StatePublished - Jul 15 1983

Keywords

  • IgG
  • albumin
  • detergents
  • electroimmunodiffusion
  • immunoprecipitation
  • quantitation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Quantitation of human immunoglobulin G and albumin in electroimmunodiffusion gels containing ionic and nonionic detergents'. Together they form a unique fingerprint.

Cite this