Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads

Gregory S. Retzinger; Bernard C. Cook; Roy E. Smith; Mary Corak McGinnis

doi:10.1016/0003-2697(91)90288-5

Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads

Gregory S. Retzinger^*, Bernard C. Cook, Roy E. Smith, Mary Corak McGinnis

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml^-1 with a precision of ±5%.

Original language	English (US)
Pages (from-to)	18-23
Number of pages	6
Journal	Analytical Biochemistry
Volume	195
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(91)90288-5
State	Published - May 15 1991

Funding

i This work was supported by Grant 89-GA-30 from the American Heart Association, Wisconsin affiliate, and by a grant from the Lucille P. Markey Foundation. G.S.R. is a Lucille P. Markey Scholar. * To whom requests for reprints should be addressed. 3 Abbreviations used: BSA, bovine serum albumin; EDTA, ethylenediaminetetraacetate; FXIIIa, factor XIIIa; SDS, sodium dodecyl sulfate; U, unit.

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads",

abstract = "Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml-1 with a precision of ±5%.",

author = "Retzinger, {Gregory S.} and Cook, {Bernard C.} and Smith, {Roy E.} and McGinnis, {Mary Corak}",

note = "Funding Information: i This work was supported by Grant 89-GA-30 from the American Heart Association, Wisconsin affiliate, and by a grant from the Lucille P. Markey Foundation. G.S.R. is a Lucille P. Markey Scholar. * To whom requests for reprints should be addressed. 3 Abbreviations used: BSA, bovine serum albumin; EDTA, ethylenediaminetetraacetate; FXIIIa, factor XIIIa; SDS, sodium dodecyl sulfate; U, unit.",

year = "1991",

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day = "15",

doi = "10.1016/0003-2697(91)90288-5",

language = "English (US)",

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pages = "18--23",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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T1 - Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads

AU - Retzinger, Gregory S.

AU - Cook, Bernard C.

AU - Smith, Roy E.

AU - McGinnis, Mary Corak

N1 - Funding Information: i This work was supported by Grant 89-GA-30 from the American Heart Association, Wisconsin affiliate, and by a grant from the Lucille P. Markey Foundation. G.S.R. is a Lucille P. Markey Scholar. * To whom requests for reprints should be addressed. 3 Abbreviations used: BSA, bovine serum albumin; EDTA, ethylenediaminetetraacetate; FXIIIa, factor XIIIa; SDS, sodium dodecyl sulfate; U, unit.

PY - 1991/5/15

Y1 - 1991/5/15

N2 - Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml-1 with a precision of ±5%.

AB - Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml-1 with a precision of ±5%.

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EP - 23

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads

Abstract

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ASJC Scopus subject areas

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