Quantitation of plasma factor XIIIa activity using fibrin-coated microscopic latex beads

Gregory S. Retzinger*, Bernard C. Cook, Roy E. Smith, Mary Corak McGinnis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Following exposure to thrombin, monodisperse microscopic polystyrene-divinylbenzene beads coated with a mixed film of lecithin and fibrinogen aggregate as a consequence of interbead fibrin polymerization. These bead aggregates rapidly dissociate in 5 m urea. Treatment of aggregates with factor XIIIa results in a dosedependent decrease of the rate of aggregate dissociation in urea. The rate of disaggregation is readily quantitated by turbidimetry. We have exploited this phenomenon to develop a rapid and sensitive method for quantitating factor XIIIa activity in plasma. Using 20 μl of human plasma the method measures the activity of factor XIIIa to a level of 0.03 U ml-1 with a precision of ±5%.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalAnalytical Biochemistry
Volume195
Issue number1
DOIs
StatePublished - May 15 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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