This study evaluates laboratory microtomography and microhardness analysis for quantifying the mineral content of bovine enamel. Fifty enamel blocks were submitted individually for 5 days to a pH-cycling model at 37°C and remained in the remineralizing solution for 2 days. The blocks were treated twice daily for l min with NaF dentifrices (Placebo, 275, 550, 1,100 μg F/g and Crest®) diluted in deionized water. Surface microhardness changes (%SMH) and mineral loss (ΔZ) were then calculated. Laboratory microtomography was also used to measure total mineral lost (LMM). Pearson's correlation (p<0.05) was used to determine the relationship between different methods of analysis and dose-response between treatments. Dentifrice fluoride concentration and %SMH and ΔZ were correlated (p<0.05). There was a positive relationship (p<0.05) when comparing LMM vs. ΔZ; a negative relationship (p<0.05) was found for %SMH vs. LMM and %SMH vs. ΔZ. Therefore, both mineral quantification techniques provide adequate precision for studying the bovine enamel-pH-cycling demineralization/remineralization model.