Quantitative analysis of mineral content in enamel using laboratory microtomography and microhardness analysis

A. E.M. Vieira*, A. C.B. Delbem, K. T. Sassaki, M. L. Cannon, S. R. Stock

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations


This study evaluates laboratory microtomography and microhardness analysis for quantifying the mineral content of bovine enamel. Fifty enamel blocks were submitted individually for 5 days to a pH-cycling model at 37°C and remained in the remineralizing solution for 2 days. The blocks were treated twice daily for l min with NaF dentifrices (Placebo, 275, 550, 1,100 μg F/g and Crest®) diluted in deionized water. Surface microhardness changes (%SMH) and mineral loss (ΔZ) were then calculated. Laboratory microtomography was also used to measure total mineral lost (LMM). Pearson's correlation (p<0.05) was used to determine the relationship between different methods of analysis and dose-response between treatments. Dentifrice fluoride concentration and %SMH and ΔZ were correlated (p<0.05). There was a positive relationship (p<0.05) when comparing LMM vs. ΔZ; a negative relationship (p<0.05) was found for %SMH vs. LMM and %SMH vs. ΔZ. Therefore, both mineral quantification techniques provide adequate precision for studying the bovine enamel-pH-cycling demineralization/remineralization model.

Original languageEnglish (US)
Title of host publicationDevelopments in X-Ray Tomography V
StatePublished - Nov 13 2006
EventDevelopments in X-Ray Tomography V - San Diego, CA, United States
Duration: Aug 15 2006Aug 17 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherDevelopments in X-Ray Tomography V
CountryUnited States
CitySan Diego, CA


  • Enamel
  • Laboratory microtomography
  • Microhardness

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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