Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy

Mark Kittisopikul; Laura Virtanen; Pekka Taimen; Robert D. Goldman

doi:10.3390/cells8040361

Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy

Mark Kittisopikul^*, Laura Virtanen, Pekka Taimen, Robert D. Goldman

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Review article › peer-review

13 Scopus citations

Abstract

The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.

Original language	English (US)
Article number	361
Journal	Cells
Volume	8
Issue number	4
DOIs	https://doi.org/10.3390/cells8040361
State	Published - Apr 2019

Keywords

Computational geometry
Delaunay triangulation
Lamins
Single molecule localization microscopy
Steerable filters
Structured illumination microscopy
Voronoi tessellation

ASJC Scopus subject areas

General Medicine

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10.3390/cells8040361

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title = "Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy",

abstract = "The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.",

keywords = "Computational geometry, Delaunay triangulation, Lamins, Single molecule localization microscopy, Steerable filters, Structured illumination microscopy, Voronoi tessellation",

author = "Mark Kittisopikul and Laura Virtanen and Pekka Taimen and Goldman, {Robert D.}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = apr,

doi = "10.3390/cells8040361",

language = "English (US)",

volume = "8",

journal = "Cells",

issn = "2073-4409",

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T1 - Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy

AU - Kittisopikul, Mark

AU - Virtanen, Laura

AU - Taimen, Pekka

AU - Goldman, Robert D.

PY - 2019/4

Y1 - 2019/4

N2 - The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.

AB - The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.

KW - Computational geometry

KW - Delaunay triangulation

KW - Lamins

KW - Single molecule localization microscopy

KW - Steerable filters

KW - Structured illumination microscopy

KW - Voronoi tessellation

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DO - 10.3390/cells8040361

M3 - Review article

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SN - 2073-4409

VL - 8

JO - Cells

JF - Cells

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ER -

Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy

Abstract

Keywords

ASJC Scopus subject areas

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