Quantitative analysis of nuclear lamins imaged by super-resolution light microscopy

Mark Kittisopikul*, Laura Virtanen, Pekka Taimen, Robert D. Goldman

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

8 Scopus citations

Abstract

The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.

Original languageEnglish (US)
Article number361
JournalCells
Volume8
Issue number4
DOIs
StatePublished - Apr 2019

Keywords

  • Computational geometry
  • Delaunay triangulation
  • Lamins
  • Single molecule localization microscopy
  • Steerable filters
  • Structured illumination microscopy
  • Voronoi tessellation

ASJC Scopus subject areas

  • Medicine(all)

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