Quantitative analysis of zinc in rat hippocampal mossy fibers by nuclear microscopy

Binbin Zhang, Minqin Ren, Fwu Shan Sheu*, Frank Watt, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Zinc (Zn) is involved in regulating mental and motor functions of the brain. Previous approaches have determined Zn content in the brain using semi-quantitative histological methods. We present here an alternative approach to map and quantify Zn levels in the synapses from mossy fibers to CA3 region of the hippocampus. Based on the use of nuclear microscopy, which is a combination of imaging and analysis techniques encompassing scanning transmission ion microscopy (STIM), Rutherford backscattering spectrometry (RBS), and particle induced X-ray emission (PIXE), it enables quantitative elemental mapping down to the parts per million (μg/g dry weight) levels of zinc in rat hippocampal mossy fibers. Our results indicate a laminar-specific Zn concentration of 240 ± 9 μM in wet weight level (135 ± 5 μg/g dry weight) in the stratum lucidum (SL) compared to 144 ± 6 μM in wet weight level (81 ± 3 μg/g dry weight) in the stratum pyramidale (SP) and 78 ± 10 μM in wet weight level (44 ± 5 μg/g dry weight) in the stratum oriens (SO) of the hippocampus. The mossy fibers terminals in CA3 are mainly located in the SL. Hence the Zn concentration is suggested to be within this axonal presynaptic terminal system.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalNeuroscience Research
Volume74
Issue number1
DOIs
StatePublished - Sep 1 2012

Keywords

  • Hippocampal mossy fibers
  • Nuclear microscopy
  • PIXE
  • Quantitative analysis
  • RBS
  • STIM
  • Zn concentration

ASJC Scopus subject areas

  • Neuroscience(all)

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