Quantitative dynamics and spatial profile of perisomatic GABAergic input during epileptiform synchronization in the CA1 hippocampus

Ivan Marchionni, Gianmaria Maccaferri*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Perisomatic GABAergic input appears spared or even increased in intractable temporal lobe epilepsy, and has been suggested to contribute to the generation of pathological discharges. Nevertheless, its degree of functional activity during epileptiform synchronization has not been thoroughly investigated. Thus, it remains unclear how structural preservation or loss of domain-specific GABAergic input may affect the network. Here, we have taken advantage of a model of epileptiform activity . in vitro to quantify the charge transfer provided by perisomatic GABAA receptor-mediated input to CA1 pyramidal neurons during interictal-like bursts. By recording both firing in GABAergic interneurons and the charge transfer generated by unitary postsynaptic currents to target pyramidal cells, we have estimated the charge transfer that would be dynamically generated by the recruitment of the entire pool of perisomatic-targeting interneurons and the number of perisomatic-targeting interneurons that would be required to generate the experimentally observed GABAergic input. In addition, we have recorded and compared the dynamics and charge density of GABAergic input recorded at different membrane compartments such as the soma . vs. the proximal dendrite. Our results suggest that GABAA receptor-mediated perisomatic input is massively activated during burst synchronization and that its kinetic properties and charge density are similar at the soma and proximal dendrite. These functional results match structural data published by other laboratories very well and strengthen the hypothesis that the potential preservation of perisomatic GABAergic input in intractable epilepsies may be a key factor in the generation of pathological network activity.

Original languageEnglish (US)
Pages (from-to)5691-5708
Number of pages18
JournalJournal of Physiology
Volume587
Issue number23
DOIs
StatePublished - Dec 2009

ASJC Scopus subject areas

  • Physiology

Fingerprint

Dive into the research topics of 'Quantitative dynamics and spatial profile of perisomatic GABAergic input during epileptiform synchronization in the CA1 hippocampus'. Together they form a unique fingerprint.

Cite this