Quantitative image analysis of mesoscale biofilm structure with optical coherence tomography

Alex Rosenthal*, Ji Yi, Hao F Zhang, Oluwaseyi Balogun, George Wells

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

The metabolic processes supported in a biofilm are often determined by a biofilm’s mesoscale structure (10-4-10-2 meter scale), and mesoscale structure is in turn shaped by the interplay between microbial metabolism and detachment. Despite the widespread adoption of biofilm reactors for water pollution control, much is unknown regarding the relationship between mesoscale biofilm structure and process performance. Optical coherence tomography (OCT) has recently emerged as a promising technique to non-destructively capture mesoscale three-dimensional images of environmentally relevant biofilms. For this study, OCT was used to image the three dimensional structure of mixed culture biofilms enriched in a synthetic wastewater. Distinct morphological features such as pores and microcolonies were detectable as deep as 1mm within the biofilm matrix. Quantitative structural parameter values (e.g. porosity, thickness, diffusion distance) were consistent across multiple fields of view and compared favorably with images acquired by confocal laser scanning microscopy.

Original languageEnglish (US)
Title of host publication88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015
PublisherWater Environment Federation
Pages4736-4745
Number of pages10
ISBN (Electronic)9781510870468
StatePublished - Jan 1 2015
Event88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015 - Chicago, United States
Duration: Sep 26 2015Sep 30 2015

Publication series

Name88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015
Volume5

Conference

Conference88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015
CountryUnited States
CityChicago
Period9/26/159/30/15

Keywords

  • Biofilm structure
  • Imaging
  • Mesoscale
  • Microscopy
  • Optical coherence tomography

ASJC Scopus subject areas

  • Ecological Modeling
  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Water Science and Technology

Fingerprint Dive into the research topics of 'Quantitative image analysis of mesoscale biofilm structure with optical coherence tomography'. Together they form a unique fingerprint.

  • Cite this

    Rosenthal, A., Yi, J., Zhang, H. F., Balogun, O., & Wells, G. (2015). Quantitative image analysis of mesoscale biofilm structure with optical coherence tomography. In 88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015 (pp. 4736-4745). (88th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2015; Vol. 5). Water Environment Federation.