Quantitative method to track proteolytic invasion in 3D collagen

Kazumi Ebine, Christina R. Chow, Hidayatullah G. Munshi*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages161-169
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

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Keywords

  • 3D collagen gels
  • Invasion
  • MT1-MMP
  • Proteolysis
  • Proteolytic tracks

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ebine, K., Chow, C. R., & Munshi, H. G. (2019). Quantitative method to track proteolytic invasion in 3D collagen. In Methods in Molecular Biology (pp. 161-169). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8879-2_15