@inbook{59d6cd7e14d84432a7ca19600c84d117,
title = "Quantitative method to track proteolytic invasion in 3D collagen",
abstract = "Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.",
keywords = "3D collagen gels, Invasion, MT1-MMP, Proteolysis, Proteolytic tracks",
author = "Kazumi Ebine and Chow, {Christina R.} and Munshi, {Hidayatullah G.}",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-8879-2_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "161--169",
booktitle = "Methods in Molecular Biology",
}
