Quantitative method to track proteolytic invasion in 3D collagen

Kazumi Ebine, Christina R. Chow, Hidayatullah G Munshi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages161-169
Number of pages9
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Collagen
Neoplasms
Gels

Keywords

  • 3D collagen gels
  • Invasion
  • MT1-MMP
  • Proteolysis
  • Proteolytic tracks

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ebine, K., Chow, C. R., & Munshi, H. G. (2019). Quantitative method to track proteolytic invasion in 3D collagen. In Methods in Molecular Biology (pp. 161-169). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8879-2_15
Ebine, Kazumi ; Chow, Christina R. ; Munshi, Hidayatullah G. / Quantitative method to track proteolytic invasion in 3D collagen. Methods in Molecular Biology. Humana Press Inc, 2019. pp. 161-169 (Methods in Molecular Biology).
@inbook{59d6cd7e14d84432a7ca19600c84d117,
title = "Quantitative method to track proteolytic invasion in 3D collagen",
abstract = "Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.",
keywords = "3D collagen gels, Invasion, MT1-MMP, Proteolysis, Proteolytic tracks",
author = "Kazumi Ebine and Chow, {Christina R.} and Munshi, {Hidayatullah G}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-8879-2_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "161--169",
booktitle = "Methods in Molecular Biology",

}

Ebine, K, Chow, CR & Munshi, HG 2019, Quantitative method to track proteolytic invasion in 3D collagen. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1882, Humana Press Inc, pp. 161-169. https://doi.org/10.1007/978-1-4939-8879-2_15

Quantitative method to track proteolytic invasion in 3D collagen. / Ebine, Kazumi; Chow, Christina R.; Munshi, Hidayatullah G.

Methods in Molecular Biology. Humana Press Inc, 2019. p. 161-169 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Quantitative method to track proteolytic invasion in 3D collagen

AU - Ebine, Kazumi

AU - Chow, Christina R.

AU - Munshi, Hidayatullah G

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.

AB - Since many tumors are associated with a pronounced collagen-rich stromal reaction, there is increasing interest in understanding mechanisms by which cancer cells invade through the collagen barrier. Here we describe a quantitative method to track cell invasion in 3D collagen I gels. We analyze invasion by quantifying proteolytic tracks generated by invading cancer cells through a 3D collagen microenvironment. We provide a detailed protocol for this quantitative assay, which can be used to characterize signaling pathways that regulate invasion in the 3D microenvironment.

KW - 3D collagen gels

KW - Invasion

KW - MT1-MMP

KW - Proteolysis

KW - Proteolytic tracks

UR - http://www.scopus.com/inward/record.url?scp=85055671191&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055671191&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8879-2_15

DO - 10.1007/978-1-4939-8879-2_15

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 161

EP - 169

BT - Methods in Molecular Biology

PB - Humana Press Inc

ER -

Ebine K, Chow CR, Munshi HG. Quantitative method to track proteolytic invasion in 3D collagen. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 161-169. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8879-2_15