Abstract
In preparation of a clinical trial of norovirus treatment, there were concerns raised by FDA about risk of self-storage of stool from patients infected with norovirus affecting quantitative assessments of norovirus RNA. Specifically, most home freezers are frost-free and may expose the samples to multiple rounds of freeze-thaw. Stool samples collected by the study team were stored at different lengths in a frost-free freezer and at −80°C. Quantitative PCRs of norovirus were performed on all samples using the same assay. By all measures, there was no significant change in measured viral load with home storage.
| Original language | English (US) |
|---|---|
| Article number | e13826 |
| Journal | Transplant Infectious Disease |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2022 |
Funding
The authors want to thank (Susan Brietigam, Michelle Callegari, Zelene Figueroa, Leah Goudy, Katrina Martin, and Kelly Memmer) for supporting the clinical study at Northwestern University Feinberg School of Medicine. They want to thank their NIH team (Rudy Alarcon, Gabi Feolo, Steve Huang and Swee Teo) for ongoing advice and support for the contracted study. The study was funded as part of NIAID contract: HHSN273301600016C. Resources supporting the trial were also provided by NCATS grant UL1TR001422. The authors want to thank (Susan Brietigam, Michelle Callegari, Zelene Figueroa, Leah Goudy, Katrina Martin, and Kelly Memmer) for supporting the clinical study at Northwestern University Feinberg School of Medicine. They want to thank their NIH team (Rudy Alarcon, Gabi Feolo, Steve Huang and Swee Teo) for ongoing advice and support for the contracted study. The study was funded as part of NIAID contract: HHSN273301600016C. Resources supporting the trial were also provided by NCATS grant UL1TR001422.
Keywords
- frost-free refrigerator
- home storage
- norovirus
- quantitative viral load
- stability
ASJC Scopus subject areas
- Transplantation
- Infectious Diseases
