Abstract
Bone marrow-derived human mesenchymal stem cells (BM-hMSCs) show promise as cell-based delivery vehicles for anti-glioma therapeutics, due to innate tropism for gliomas. However, in clinically relevant human-in-mouse glioma stem cell xenograft models, BM-hMSCs tropism is variable. We compared the proteomic profile of cancer and stromal cells in GSCXs that attract BM-hMSCs ("attractors") with those to do not ("non-attractors") to identify pathways that may modulate BM-hMSC homing, followed by targeted transcriptomics. The results provide the first link between fatty acid metabolism, glucose metabolism, ROS, and N-glycosylation patterns in attractors. Reciprocal expression of these pathways in the stromal cells suggests microenvironmental cross-talk.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 94-103 |
| Number of pages | 10 |
| Journal | EuPA Open Proteomics |
| Volume | 8 |
| DOIs | |
| State | Published - Sep 1 2015 |
Funding
The authors gratefully acknowledge the financial support of the Cancer Prevention Research Institute of Texas (CPRIT) and The University of Texas Medical Branch to C.L.N. as well as the Dr. Ralph and Marian Falk Medical Research Trust to J.R.M, are gratefully acknowledged. The authors would like to thank Dr. Ekaterina Mostovenko for the R script for visualizing log 2 -transformed expression values as hexadecimal color codes. The authors gratefully acknowledge Dr. Frederick F. Lang and Joy Gumin for the glioma stem cell xenografts (GSCXs) at MD Anderson Cancer Center for this study.
Keywords
- Bone marrow-derived human mesenchymal stem cells (BM-hMSCs)
- Cancer proteomics
- Fatty acid metabolism
- Glioblastoma
- Glycolysis
- Glycosylation
- Mass spectrometry
- Pentose phosphate pathway
- ROS
- Transcriptomics
ASJC Scopus subject areas
- Biochemistry
