Quantitative two-site enzyme-linked immunosorbent assays for inhibin A, activin A and activin B

T. Woodruff*, L. Krummen, D. Baly, S. Garg, D. Allison, M. Sadick, W. Wong, J. Mather, M. Soules

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We have developed three specific enzyme-linked immunosorbentassay (ELISA) formats which quantitate inhibin Ain conditioned media and serum.The assays are sensitive ina range 0.078—5.0 ng/ml and have been characterized interms of cross-reactivity to inhibin related proteins.The CK:CK assay format recognizes inhibin A, inhibin B and inhibin-related molecules, while the 9A9:CK assay format recognizes inhibin A and inhibin A precursors, but not freea-subunit.The 11B5:CK assay appears to recognize only mature32 kDa inhibin A.Additionally, we have developed separate, specific ELISA formats which quantitate activin A and activin B.The assays have a range of 0.2-50 ng/ml and 0.4-50 ng/ml for activin A and recombinant activin B, respectively.These assays are presently being used to examine th concentration of inhibin A, activin A and activin B in clinical serum samples.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalHuman Reproduction
Volume8
DOIs
StatePublished - Nov 1993

Keywords

  • Activin
  • Bioassay
  • Elisa
  • Inhibin
  • Quantitation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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