R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons

Alexia E. Metz, Tim Jarsky, Marco Martina, Nelson Spruston*

*Corresponding author for this work

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Action potentials in pyramidal neurons are typically followed by an afterdepolarization (ADP), which in many cells contributes to intrinsic burst firing. Despite the ubiquity of this common excitable property, the responsible ion channels have not been identified. Using current-clamp recordings in hippocampal slices, we find that the ADP in CA1 pyramidal neurons is mediated by an Ni2+-sensitive calcium tail current. Voltage-clamp experiments indicate that the Ni2+-sensitive current has a pharmacological and biophysical profile consistent with R-type calcium channels. These channels are available at the resting potential, are activated by the action potential, and remain open long enough to drive the ADP. Because the ADP correlates directly with burst firing in CA1 neurons, R-type calcium channels are crucial to this important cellular behavior, which is known to encode hippocampal place fields and enhance synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)5763-5773
Number of pages11
JournalJournal of Neuroscience
Volume25
Issue number24
DOIs
StatePublished - Jun 15 2005

Fingerprint

R-Type Calcium Channels
Pyramidal Cells
Action Potentials
Neuronal Plasticity
Ion Channels
Membrane Potentials
Pharmacology
Calcium
Neurons

Keywords

  • ADP
  • Afterhyperpolarization
  • Intrinsic excitability
  • Nickel
  • Patch clamp
  • Persistent sodium current

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Metz, Alexia E. ; Jarsky, Tim ; Martina, Marco ; Spruston, Nelson. / R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 24. pp. 5763-5773.
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R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons. / Metz, Alexia E.; Jarsky, Tim; Martina, Marco; Spruston, Nelson.

In: Journal of Neuroscience, Vol. 25, No. 24, 15.06.2005, p. 5763-5773.

Research output: Contribution to journalArticle

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T1 - R-type calcium channels contribute to afterdepolarization and bursting in hippocampal CA1 pyramidal neurons

AU - Metz, Alexia E.

AU - Jarsky, Tim

AU - Martina, Marco

AU - Spruston, Nelson

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AB - Action potentials in pyramidal neurons are typically followed by an afterdepolarization (ADP), which in many cells contributes to intrinsic burst firing. Despite the ubiquity of this common excitable property, the responsible ion channels have not been identified. Using current-clamp recordings in hippocampal slices, we find that the ADP in CA1 pyramidal neurons is mediated by an Ni2+-sensitive calcium tail current. Voltage-clamp experiments indicate that the Ni2+-sensitive current has a pharmacological and biophysical profile consistent with R-type calcium channels. These channels are available at the resting potential, are activated by the action potential, and remain open long enough to drive the ADP. Because the ADP correlates directly with burst firing in CA1 neurons, R-type calcium channels are crucial to this important cellular behavior, which is known to encode hippocampal place fields and enhance synaptic plasticity.

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