R5 and X4 HIV envelopes induce distinct gene expression profiles in primary peripheral blood mononuclear cells

Claudia Cicala*, James Arthos, Elena Martinelli, Nina Censoplano, Catherine C. Cruz, Eva Chung, Sara M. Selig, Donald Van Ryk, Jun Yang, Shyla Jagannatha, Tae Wook Chun, Ping Ren, Richard A. Lempicki, Anthony S. Fauci

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


HIV envelope binds to and signals through its primary cellular receptor, CD4, and through a coreceptor, either CC chemokine receptor 5 (CCR5) or CXC chemokine receptor 4 (CXCR4). Here, we evaluate the response of peripheral blood mononuclear cells to a panel of genetically diverse R5 and X4 envelope proteins. Modulation of gene expression was evaluated by using oligonucleotide microarrays. Activation of transcription factors was evaluated by using an array of oligonucleotides encoding transcription factor binding sites. Responses were strongly influenced by coreceptor specificity. Treatment of cells from CCR5AΔ32 homozygous donors with glycoprotein (gp)120 derived from an R5 virus demonstrated that the majority of responses elicited by R5 envelopes required engagement of CCR5. RS envelopes, to a greater extent than X4 envelopes, induced the expression of genes belonging to mitogen-activated protein kinase signal transduction pathways and genes regulating the cell cycle. A number of genes induced by R5, but not X4, envelopes were also up-regulated in the resting CD4+ T cell population of HIV-infected individuals. These results suggest that R5 envelope facilitates replication of HIV in the pool of resting CD4+ T cells. Additionally, signaling by R5 gp120 may facilitate the transmission of R5 viruses by inducing a permissive environment for HIV replication.

Original languageEnglish (US)
Pages (from-to)3746-3751
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - Mar 7 2006
Externally publishedYes


  • Glycoprotein 120
  • Microarray
  • Signal transduction
  • Tropism
  • Viral replication

ASJC Scopus subject areas

  • General


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