R7BP augments the function of RGS7·Gβ5 complexes by a plasma membrane-targeting mechanism

Ryan M. Drenan, Craig A. Doupnik, Muralidharan Jayaraman, Abigail L. Buchwalter, Kevin M. Kaltenbronn, James E. Huettner, Maurine E. Linder, Kendall J. Blumer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


The RGS7 (R7) family of G protein regulators, Gβ5, and R7BP form heterotrimeric complexes that potently regulate the kinetics of G protein-coupled receptor signaling. Reversible palmitoylation of R7BP regulates plasma membrane/nuclear shuttling of R7·Gβ5·R7BP heterotrimers. Here we have investigated mechanisms whereby R7BP controls the function of the R7 family. We show that unpalmitoylated R7BP undergoes nuclear/cytoplasmic shuttling and that a C-terminal polybasic motif proximal to the palmitoylation acceptor sites of R7BP mediates nuclear localization, palmitoylation, and plasma membrane targeting. These results suggest a novel mechanism whereby palmitoyltransferases and nuclear import receptors both utilize the C-terminal domain of R7BP to determine the trafficking fate of R7·Gβ5·R7BP heterotrimers. Analogous mechanisms may regulate other signaling proteins whose distribution between the plasma membrane and nucleus is controlled by palmitoylation. Lastly, we show that cytoplasmic RGS7·Gβ5·R7BP heterotrimers and RGS7·Gβ5 heterodimers are equivalently inefficient regulators of G protein-coupled receptor signaling relative to plasma membrane-bound heterotrimers bearing palmitoylated R7BP. Therefore, R7BP augments the function of the complex by a palmitoylation-regulated plasma membrane-targeting mechanism.

Original languageEnglish (US)
Pages (from-to)28222-28231
Number of pages10
JournalJournal of Biological Chemistry
Issue number38
StatePublished - Sep 22 2006

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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