Molecular genetics studies have recently revealed complexity in the MHC class II region that has not been detected by previous serologic and genetic studies. In humans, three subregions, DP, DQ, and DR, of the class II genes as well as the DZα and DOβ genes, have been extensively characterized. Although homologs of these human genes were identified in many species, their expressibility has not been well defined in species other than the mouse. We have previously cloned the rabbit homologs of the HLA-DPα and β genes whose protein products had never been detected. The sequences of rabbit DPα1 and DPβ genes are reported herein and they indicate that the rabbit DP genes encode functional α- and β-chains. Unfavorable nucleotides surrounding the first AUG codon may, however, reduce the translational efficiency of the R-DPβ mRNA and explain the difficulty in generating serologic reagents specific for rabbit DP molecules. A complex mutation in the β1 domain of the R-DPβ gene was similar to the one found in the H-2Aβ1 gene of five strains of mice. The origin of this mutation is discussed.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Immunology|
|State||Published - 1988|
ASJC Scopus subject areas
- Immunology and Allergy