Racial differences in prostate cancer related to loss of heterozygosity on chromosome 8p12-23

John A. Kalapurakal, A. N.K. Jacob, Philip Y. Kim, Denise D. Najjar, Yi C. Hsieh, Philip Ginsberg, Ierachmiel Daskal, Sucha O. Asbell, Rajendra P. Kandpal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Purpose: To determine if there is a racial difference in prostate cancer related to loss of heterozygosity (LOH) on chromosome 8p12-23, the region most frequently altered in prostate cancer. Methods and Materials: A total of 51 prostate cancer patients, consisting of 23 African Americans and 28 Caucasians, were included in this study. All patients underwent radical prostatectomy, and patients in the two racial subgroups were matched for median serum PSA, Gleason score, and pathological stage of cancer. Paired normal prostate and cancer tissue DNA was isolated and amplified with 13 polymorphic markers mapped to 8p12-23 by radiolabeled polymerase chain reaction. The amplified products were resolved by polyacrylamide gel electrophoresis, autoradiographed, and analyzed for allelic losses.Results: The overall incidence of LOH at 8p12-23 was 53%, and 16% showed homozygous deletions. The incidence of LOH in Caucasians was 68% compared to 35% in African Americans. On univariate (p = 0.02) and multivariate logistic regression analysis (p = 0.02), only Caucasian race was a significant predictor for LOH. The other clinicopathologic parameters did not have any significant effect on incidence of LOH.Conclusion: These results highlight the independent influence of Caucasian race on incidence of LOH at 8p12-23, and suggest that genetic differences at specific tumor suppressor loci may be a factor responsible for racial variations observed in prostate cancer. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)835-840
Number of pages6
JournalInternational Journal of Radiation Oncology Biology Physics
Volume45
Issue number4
DOIs
StatePublished - Nov 1 1999

Keywords

  • Chromosome 8p
  • Loss of heterozygosity
  • Prostate cancer
  • Race
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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