Radiation survival of human mammary carcinoma cells: Criteria for an agar-based clonogenic assay

Greta J. Besch; Martin A. Tanner; Carol A. Sattler; William H. Wolberg; Steven P. Howar; Michael N. Gould

doi:10.1016/0360-3016(86)90418-9

Radiation survival of human mammary carcinoma cells: Criteria for an agar-based clonogenic assay

Greta J. Besch, Martin A. Tanner, Carol A. Sattler, William H. Wolberg, Steven P. Howar, Michael N. Gould^*

^*Corresponding author for this work

Statistics and Data Science

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Abstract

A human in vivo-in vitro model for breast cancer has been developed based on culture methods that were systematically optimized for solid breast carcinomas. A V-79 cell clonal agar-based model was used to define the analysis problem encountered with agar based clonogenic survival assays. Based on this, we established methods for the generation and analysis of the survival of primary and recurrent breast carcinomas following irradiation. Four out of five primary breast carcinomas studied had similar radiation-cell survival curves. The fifth tumor was more radioresistant. Interestingly, a recurrent breast carcinoma arising in a heavily irradiated chest wall was no more radioresistant than our series of unirradiated primary carcinomas. These methods may be useful both for the study of the radiobiology of human neoplasms and for customizing the treatments and prognosis of individual patients.

Original language	English (US)
Pages (from-to)	75-81
Number of pages	7
Journal	International journal of radiation oncology, biology, physics
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/0360-3016(86)90418-9
State	Published - Jan 1986

Keywords

Breast cancer
In vitro
Radiation
Recurrent cancer

ASJC Scopus subject areas

Radiation
Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0360-3016(86)90418-9

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@article{79265ef482bd43358343f9b161398276,

title = "Radiation survival of human mammary carcinoma cells: Criteria for an agar-based clonogenic assay",

abstract = "A human in vivo-in vitro model for breast cancer has been developed based on culture methods that were systematically optimized for solid breast carcinomas. A V-79 cell clonal agar-based model was used to define the analysis problem encountered with agar based clonogenic survival assays. Based on this, we established methods for the generation and analysis of the survival of primary and recurrent breast carcinomas following irradiation. Four out of five primary breast carcinomas studied had similar radiation-cell survival curves. The fifth tumor was more radioresistant. Interestingly, a recurrent breast carcinoma arising in a heavily irradiated chest wall was no more radioresistant than our series of unirradiated primary carcinomas. These methods may be useful both for the study of the radiobiology of human neoplasms and for customizing the treatments and prognosis of individual patients.",

keywords = "Breast cancer, In vitro, Radiation, Recurrent cancer",

author = "Besch, {Greta J.} and Tanner, {Martin A.} and Sattler, {Carol A.} and Wolberg, {William H.} and Howar, {Steven P.} and Gould, {Michael N.}",

note = "Funding Information: Work supported in part by PHS, NIH, NC1 grant numbers CA20432, CA07 175 and CA35464 and by the Wendy Will Case Cancer Fund. GJB and SPH are predoctoral trainees supported by grant numbers 5T32-CA09206 and 1T32-CA0947 1.",

year = "1986",

month = jan,

doi = "10.1016/0360-3016(86)90418-9",

language = "English (US)",

volume = "12",

pages = "75--81",

journal = "International Journal of Radiation Oncology Biology Physics",

issn = "0360-3016",

publisher = "Elsevier Inc.",

number = "1",

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TY - JOUR

T1 - Radiation survival of human mammary carcinoma cells

T2 - Criteria for an agar-based clonogenic assay

AU - Besch, Greta J.

AU - Tanner, Martin A.

AU - Sattler, Carol A.

AU - Wolberg, William H.

AU - Howar, Steven P.

AU - Gould, Michael N.

N1 - Funding Information: Work supported in part by PHS, NIH, NC1 grant numbers CA20432, CA07 175 and CA35464 and by the Wendy Will Case Cancer Fund. GJB and SPH are predoctoral trainees supported by grant numbers 5T32-CA09206 and 1T32-CA0947 1.

PY - 1986/1

Y1 - 1986/1

N2 - A human in vivo-in vitro model for breast cancer has been developed based on culture methods that were systematically optimized for solid breast carcinomas. A V-79 cell clonal agar-based model was used to define the analysis problem encountered with agar based clonogenic survival assays. Based on this, we established methods for the generation and analysis of the survival of primary and recurrent breast carcinomas following irradiation. Four out of five primary breast carcinomas studied had similar radiation-cell survival curves. The fifth tumor was more radioresistant. Interestingly, a recurrent breast carcinoma arising in a heavily irradiated chest wall was no more radioresistant than our series of unirradiated primary carcinomas. These methods may be useful both for the study of the radiobiology of human neoplasms and for customizing the treatments and prognosis of individual patients.

AB - A human in vivo-in vitro model for breast cancer has been developed based on culture methods that were systematically optimized for solid breast carcinomas. A V-79 cell clonal agar-based model was used to define the analysis problem encountered with agar based clonogenic survival assays. Based on this, we established methods for the generation and analysis of the survival of primary and recurrent breast carcinomas following irradiation. Four out of five primary breast carcinomas studied had similar radiation-cell survival curves. The fifth tumor was more radioresistant. Interestingly, a recurrent breast carcinoma arising in a heavily irradiated chest wall was no more radioresistant than our series of unirradiated primary carcinomas. These methods may be useful both for the study of the radiobiology of human neoplasms and for customizing the treatments and prognosis of individual patients.

KW - Breast cancer

KW - In vitro

KW - Radiation

KW - Recurrent cancer

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SN - 0360-3016

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EP - 81

JO - International Journal of Radiation Oncology Biology Physics

JF - International Journal of Radiation Oncology Biology Physics

IS - 1

ER -

Radiation survival of human mammary carcinoma cells: Criteria for an agar-based clonogenic assay

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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