Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection

Khalief E. Hamden; Patrick W. Ford; Audy G. Whitman; Ossie F. Dyson; Shi Yuan Cheng; James A. McCubrey; Shaw M. Akula

doi:10.1128/JVI.78.23.13381-13390.2004

Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection

Khalief E. Hamden, Patrick W. Ford, Audy G. Whitman, Ossie F. Dyson, Shi Yuan Cheng, James A. McCubrey, Shaw M. Akula^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf_[FF]:ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of IIFF/ΔB-Raf_[DD]:ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf_[DD]:ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF¹²¹. These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells.

Original language	English (US)
Pages (from-to)	13381-13390
Number of pages	10
Journal	Journal of virology
Volume	78
Issue number	23
DOIs	https://doi.org/10.1128/JVI.78.23.13381-13390.2004
State	Published - Dec 2004

Funding

ASJC Scopus subject areas

Insect Science
Virology
Microbiology
Immunology

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10.1128/JVI.78.23.13381-13390.2004

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title = "Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection",

abstract = "Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf[FF]:ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of IIFF/ΔB-Raf[DD]:ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf[DD]:ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF121. These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells.",

author = "Hamden, {Khalief E.} and Ford, {Patrick W.} and Whitman, {Audy G.} and Dyson, {Ossie F.} and Cheng, {Shi Yuan} and McCubrey, {James A.} and Akula, {Shaw M.}",

year = "2004",

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doi = "10.1128/JVI.78.23.13381-13390.2004",

language = "English (US)",

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T1 - Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection

AU - Hamden, Khalief E.

AU - Ford, Patrick W.

AU - Whitman, Audy G.

AU - Dyson, Ossie F.

AU - Cheng, Shi Yuan

AU - McCubrey, James A.

AU - Akula, Shaw M.

PY - 2004/12

Y1 - 2004/12

N2 - Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf[FF]:ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of IIFF/ΔB-Raf[DD]:ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf[DD]:ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF121. These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells.

AB - Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf[FF]:ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of IIFF/ΔB-Raf[DD]:ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf[DD]:ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF121. These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells.

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Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection

Abstract

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ASJC Scopus subject areas

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