Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection

Khalief E. Hamden, Patrick W. Ford, Audy G. Whitman, Ossie F. Dyson, Shi Yuan Cheng, James A. McCubrey, Shaw M. Akula*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Recombinant green fluorescent protein encoding Kaposi's sarcoma-associated herpesvirus (rKSHV.152) infection of β-estradiol stimulated human foreskin fibroblasts (HFF) or HFF/ΔB-Raf[FF]:ER (expressing a weaker form of B-Raf) could be enhanced to levels comparable to that of IIFF/ΔB-Raf[DD]:ER cells by pretreating cells with soluble vascular endothelial growth factor (VEGF). Conversely, VEGF expression and infection efficiency typically observed in β-estradiol stimulated HFF/ΔB-Raf[DD]:ER cells could be lowered significantly by treating with VEGF small interfering RNA. In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF121. These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells.

Original languageEnglish (US)
Pages (from-to)13381-13390
Number of pages10
JournalJournal of virology
Issue number23
StatePublished - Dec 2004

ASJC Scopus subject areas

  • Insect Science
  • Virology
  • Microbiology
  • Immunology


Dive into the research topics of 'Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection'. Together they form a unique fingerprint.

Cite this