Random-Access Multiphoton Microscopy for Fast Three-Dimensional Imaging

Gaddum Duemani Reddy, Ronald J. Cotton, Andreas S. Tolias, Peter Saggau*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Studies in several important areas of neuroscience, including analysis of single neurons as well as neural networks, continue to be limited by currently available experimental tools. By combining molecular probes of cellular function, such as voltage-sensitive or calcium-sensitive dyes, with advanced microscopy techniques such as multiphoton microscopy, experimental neurophysiologists have been able to partially reduce this limitation. These approaches usually provide the needed spatial resolution along with convenient optical sectioning capabilities for isolating regions of interest. However, they often fall short in providing the necessary temporal resolution, primarily due to their restrained laser scanning mechanisms. In this regard, we review a method of laser scanning for multiphoton microscopy that overcomes the temporal limitations of pervious approaches and allows for what is known as 3D Random Access Multiphoton (3D RAMP) microscopy, an imaging technique that supports full three dimensional recording of many sites of interest on physiologically relevant time scales.

Original languageEnglish (US)
Pages (from-to)455-472
Number of pages18
JournalAdvances in experimental medicine and biology
StatePublished - 2015
Externally publishedYes


  • Acousto-optic deflector
  • Di 8 ANEPPS
  • Microscopy
  • Motion tracking
  • Multiphoton
  • Random-access scanning
  • Spatial dispersion
  • Temporal dispersion
  • Three-dimensional

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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