Rantes chemokine expression in diseased and normal human tissues

Irene Von Luettichau, Peter J. Nelson, James M. Pattison, Matt Van De Rijn, Phil Huie, Roger Warnke, Christian J. Wiedermann, Rolf A K Stahl, Richard K. Sibley, Alan M. Krensky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

RANTES is a member of a large family of cytokines, called chemokines, which are thought to play a regulatory role in inflammatory processes. We have made recombinant human RANTES protein which was used to generate a panel of anti-RANTES monoclonal antibodies. Following characterization, select anti-RANTES monoclonal antibodies were used for immunohistologic staining of a large panel of normal, diseased and fetal tissue sections. Diseased tissues included eleven lymphomas and eight renal tumors. Most tissues were also tested in parallel for RANTES mRNA by in situ hybridization using RANTES mRNA specific oligomeric probes. As expected, most normal adult tissues contain few if any, RANTES positive cells. In constrast, RANTES expression dramatically increases in inflammatory sites. In addition, megakaryocytes, some tumours, and select fetal tissues express high levels of RANTES message and protein. These results indicate a wider expression of RANTES than previously appreciated and suggest multiple physiologic roles for this soluble factor.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalCytokine
Volume8
Issue number1
DOIs
StatePublished - Jan 1996

Keywords

  • Chemokine
  • Development
  • Disease
  • Mono-clonal antibodies
  • RANTES

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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