Rapid continuous electrical lysis of bacteria on structured electrodes preserves RNA integrity

Mahla Poudineh*, Reza M. Mohamadi, Andrew Sage, Laili Mahmoudian, Edward H. Sargent, Shana O. Kelley

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a rapid electrical lysis method using structured gold electrodes in a microfluidic device. This strategy is capable of efficient and high throughput lysis of bacteria to prepare intracellular genomic materials for subsequent analysis. High electrical field at the tips of structured gold electrodes eliminates the requirement of high voltage. We have used AC voltage for lysis, and shown at low frequency; lysis would be electrochemical while at high frequency it occurs due to the electrical field.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1178-1180
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Electrical lysis
  • RNA integrity
  • Structured electrodes

ASJC Scopus subject areas

  • Control and Systems Engineering

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