Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography

Patricia A. O'Hern*, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.

Original languageEnglish (US)
Pages (from-to)59-62
Number of pages4
JournalProtein Expression and Purification
Volume2
Issue number1
DOIs
StatePublished - Feb 1991

ASJC Scopus subject areas

  • Biotechnology

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