Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography

Patricia A. O'Hern; Erwin Goldberg

doi:10.1016/1046-5928(91)90010-G

Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography

Patricia A. O'Hern^*, Erwin Goldberg

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.

Original language	English (US)
Pages (from-to)	59-62
Number of pages	4
Journal	Protein Expression and Purification
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/1046-5928(91)90010-G
State	Published - Feb 1991

Funding

We thank Dereck Wood for his excellent technical assistance on this project. This work was supported by Grant HD23771 to E.G. from the National Institute of Child Health and Human Development.

ASJC Scopus subject areas

Biotechnology

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10.1016/1046-5928(91)90010-G

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title = "Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography",

abstract = "Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.",

author = "O'Hern, {Patricia A.} and Erwin Goldberg",

note = "Funding Information: We thank Dereck Wood for his excellent technical assistance on this project. This work was supported by Grant HD23771 to E.G. from the National Institute of Child Health and Human Development.",

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AU - O'Hern, Patricia A.

AU - Goldberg, Erwin

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PY - 1991/2

Y1 - 1991/2

N2 - Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.

AB - Open-air hydrophobic interaction chromatography with alkyl carbon functional groups coupled to agarose beads has been used to desalt large volumes of ammonium sulfate-fractionated bacterial cell lysates. The protein of interest can be simultaneously desalted and concentrated in less than 4 h, and the yield is significantly better than that obtained by the standard technique of precipitation, centrifugation, and dialysis.

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Rapid desalting of ammonium sulfate solutions by hydrophobic interaction chromatography

Abstract

Funding

ASJC Scopus subject areas

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