Rapid determination of the rate constant of agonist-induced association of single platelets

Lawrence L. Hause; Gregory S. Retzinger; Patricia A. Megan

doi:10.1016/0049-3848(91)90237-Q

Rapid determination of the rate constant of agonist-induced association of single platelets

Lawrence L. Hause^*, Gregory S. Retzinger, Patricia A. Megan

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis. A linear relationship between initial platelet concentration and the rate constant of association is defined. The clinical utility of this association parameter is discussed.

Original language	English (US)
Pages (from-to)	105-112
Number of pages	8
Journal	Thrombosis research
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/0049-3848(91)90237-Q
State	Published - Jan 15 1991

Funding

This work was supported in part by research funds from the Department of Pathology, Medical College of Wisconsin, by grant 89-GA-30 from the Wisconsin affiliate of the American Heart Association, and by a grant from the Lucille P. Markey Foundation. G.S. Retzinger is a Lucille P. Markey Scholar. We thank Pamela Sweet for preparation of the manuscript.

Keywords

kinetics
platelet aggregation
platelet association

ASJC Scopus subject areas

Hematology

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10.1016/0049-3848(91)90237-Q

Cite this

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title = "Rapid determination of the rate constant of agonist-induced association of single platelets",

abstract = "We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis. A linear relationship between initial platelet concentration and the rate constant of association is defined. The clinical utility of this association parameter is discussed.",

keywords = "kinetics, platelet aggregation, platelet association",

author = "Hause, {Lawrence L.} and Retzinger, {Gregory S.} and Megan, {Patricia A.}",

note = "Funding Information: This work was supported in part by research funds from the Department of Pathology, Medical College of Wisconsin, by grant 89-GA-30 from the Wisconsin affiliate of the American Heart Association, and by a grant from the Lucille P. Markey Foundation. G.S. Retzinger is a Lucille P. Markey Scholar. We thank Pamela Sweet for preparation of the manuscript.",

year = "1991",

month = jan,

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doi = "10.1016/0049-3848(91)90237-Q",

language = "English (US)",

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journal = "Thrombosis research",

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T1 - Rapid determination of the rate constant of agonist-induced association of single platelets

AU - Hause, Lawrence L.

AU - Retzinger, Gregory S.

AU - Megan, Patricia A.

N1 - Funding Information: This work was supported in part by research funds from the Department of Pathology, Medical College of Wisconsin, by grant 89-GA-30 from the Wisconsin affiliate of the American Heart Association, and by a grant from the Lucille P. Markey Foundation. G.S. Retzinger is a Lucille P. Markey Scholar. We thank Pamela Sweet for preparation of the manuscript.

PY - 1991/1/15

Y1 - 1991/1/15

N2 - We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis. A linear relationship between initial platelet concentration and the rate constant of association is defined. The clinical utility of this association parameter is discussed.

AB - We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis. A linear relationship between initial platelet concentration and the rate constant of association is defined. The clinical utility of this association parameter is discussed.

KW - kinetics

KW - platelet aggregation

KW - platelet association

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JO - Thrombosis research

JF - Thrombosis research

IS - 2

ER -

Rapid determination of the rate constant of agonist-induced association of single platelets

Abstract

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