Abstract
Using optogenetics, the MS2-MCP system, and ChIP-seq techniques, Keenan et al. investigate the dynamic transcriptional response of ERK signaling in the early Drosophila embryo. They show that ERK signaling relieves repression of genes on a short timescale and that repression can return when signal is removed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 794-801.e4 |
| Journal | Developmental Cell |
| Volume | 52 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 23 2020 |
Funding
We are grateful to members of the Shvartsman and Wieschaus laboratories for feedback and suggestions. We thank S. McFann for advice on optogenetic experiments. We thank L. Yang for providing preliminary information on the concentration of Cic in the nucleus and assistance with ChIP-seq experiments. We thank G. Jimenez, T. Schüpbach, Alexey Veraksa, M. Levine, A. Sharrocks, J. Toettcher, Y. Goyal, and H. Johnson for helpful discussions and reagents. We thank G. Laevsky from the Princeton Nikon Imaging Facility for assistance with microscopy and thank the staff of the Sequencing Core Facility of the Lewis-Sigler Institute for help with the ChIP-seq experiments. This work was supported by NIH grant HD085870 . We are grateful to members of the Shvartsman and Wieschaus laboratories for feedback and suggestions. We thank S. McFann for advice on optogenetic experiments. We thank L. Yang for providing preliminary information on the concentration of Cic in the nucleus and assistance with ChIP-seq experiments. We thank G. Jimenez, T. Sch?pbach, Alexey Veraksa, M. Levine, A. Sharrocks, J. Toettcher, Y. Goyal, and H. Johnson for helpful discussions and reagents. We thank G. Laevsky from the Princeton Nikon Imaging Facility for assistance with microscopy and thank the staff of the Sequencing Core Facility of the Lewis-Sigler Institute for help with the ChIP-seq experiments. This work was supported by NIH grant HD085870. S.E.K. S.A.B. E.F.W. and S.Y.S. generated the ideas and designed the experiments of the study. S.E.K. conducted experiments and analyzed data. R.A.M. generated the transgenic line with endogenously tagged Cic. N.J.-V.D. generated the transgenic line with tll-MS2. S.E.K. E.F.W. and S.Y.S. wrote the manuscript with input from S.A.B. and R.A.M. The authors declare no competing interests.
Keywords
- Capicua
- Drosophila
- ERK
- cellular signaling
- development
- optogenetics
- transcriptional repression
ASJC Scopus subject areas
- Molecular Biology
- General Biochemistry, Genetics and Molecular Biology
- Developmental Biology
- Cell Biology