Rapid induction of carcinomas and γ-glutamyl transpeptidase-rich clones in N-methyl-N-benzylnitrosamine-treated hamster buccal pouch

D. B. Solt, L. Calderon-Solt, T. Odajima

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Topical application of N-methyl-N-benzylnitrosamine[(MBN) CAS: 937-40-61] to Syrian hamster buccal pouch epithelia resulted within 4 days in the induction of epithelial cell foci with histochemical γ-glutamyl transpeptidase (GGTase) activity. Approximately 94% of the foci consisted of individual cells or nests of 8 cells or less, as visualized in wholemounts of GGTase-stained pouch epithelia. During a regimen of twice weekly MBN applications, the GGTase-stained foci increased in size to a maximum diameter of approximately 0.4 mm by the third week. In histologic sections, the enlarging foci were similar morphologically to those of the adjacent GGTase-negative epithelium. Groups of animals treated with MBN for 2, 4, and 8 weeks developed a buccal pouch tumor incidence of 43, 86, and 100%, respectively, within 39 weeks or less. Of the 138 papillomas and carcinomas examined, 11 contained patchy GTase activity.

Original languageEnglish (US)
Pages (from-to)437-445
Number of pages9
JournalJournal of the National Cancer Institute
Volume74
Issue number2
StatePublished - 1985

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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