Rapid miniaturized chromatography for 111In labeled monoclonal antibodies: Comparison to size exclusion high performance liquid chromatography

A. Michael Zimmer*, Joanne M. Kazikiewicz, Stewart M. Spies, Steven T. Rosen

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Our laboratory investigated the use of a rapid miniaturized chromatography system, ITLC-SG with 0.9% NaCl, to assess the radiochemical purity of 111In labeled monoclonal antibodies (MoAbs). Radiochemical analysis was performed on numerous 111In labeled antibody preparations with labeling efficiencies ranging from 40 to greater than 95% and the results compared to those obtained with size exclusion high performance liquid chromatography (HPLC). The chromatographic procedure involved challenging radiolabeled antibodies with 0.05 M DTPA to chelate unbound and/or non-specific bound 111In, spotting on miniaturized instant thin layer-silica gel chromatography strips, developing in 0.9% NaCl, and counting appropriate segments for radioactivity. Results of the study demonstrated that the miniaturized chromatography procedure was rapid, taking less than 4 min to complete, and accurate in assessing the amount of unbound or non-specific bound 111In in 111In labeled monoclonal antibodies, when compared to size exclusion HPLC.

Original languageEnglish (US)
Pages (from-to)717-720
Number of pages4
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume15
Issue number6
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Medicine(all)

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