@inproceedings{02bfa51f174048f782bb690293a4f60d,
title = "Rapid on-chip lysis and ultrasensitive electrochemical detection of bacteria",
abstract = "Rapid detection of low concentrations of bacterial mRNA using on-chip sensors is challenging as mRNA molecules accumulate slowly at the sensor. We overcome this barrier using a novel device capable of bacterial lysis and detection of released mRNA. The chip is patterned with an array of wells each of which contains electrical lysis electrodes situated 50 microns from an electrochemical nucleic acid sensor. Lysing releases the intracellular mRNA which quickly diffuses to the nearby in-well sensor. We demonstrate rapid and sensitive detection of mRNA from E. coli within 30 minutes.",
keywords = "Bacterial detection, Cell lysis, Electrochemical sensors, Microelectrodes",
author = "Besant, {J. D.} and J. Das and Sargent, {E. H.} and Kelley, {S. O.}",
year = "2014",
language = "English (US)",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "230--232",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
}