Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (105) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of the American Chemical Society|
|State||Published - Jun 1 1994|
ASJC Scopus subject areas
- Colloid and Surface Chemistry