Rapid Sequencing of Oligonucleotides by High-Resolution Mass Spectrometry

Daniel P. Little, Russell A. Chorush, J. Paul Speir, Michael W. Senko, Neil L. Kelleher, Fred W. McLafferty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (105) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

Original languageEnglish (US)
Pages (from-to)4893-4897
Number of pages5
JournalJournal of the American Chemical Society
Issue number11
StatePublished - Jun 1 1994

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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