Rapid Sequencing of Oligonucleotides by High-Resolution Mass Spectrometry

Daniel P. Little; Russell A. Chorush; J. Paul Speir; Michael W. Senko; Neil L. Kelleher; Fred W. McLafferty

doi:10.1021/ja00090a039

Rapid Sequencing of Oligonucleotides by High-Resolution Mass Spectrometry

Daniel P. Little, Russell A. Chorush, J. Paul Speir, Michael W. Senko, Neil L. Kelleher, Fred W. McLafferty^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (10⁵) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

Original language	English (US)
Pages (from-to)	4893-4897
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	116
Issue number	11
DOIs	https://doi.org/10.1021/ja00090a039
State	Published - Jun 1 1994

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (105) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.",

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AU - Chorush, Russell A.

AU - Speir, J. Paul

AU - Senko, Michael W.

AU - Kelleher, Neil L.

AU - McLafferty, Fred W.

PY - 1994/6/1

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N2 - Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (105) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

AB - Electrospray ionization coupled with Fourier-transform mass spectrometry routinely achieves sufficiently high mass accuracy (≦20 ppm) and resolving power (105) to provide base composition and sequence information from oligonucleotides as large as 25 bases. Nozzle skimmer (NS) dissociation yields fragment anions that describe, consistent with the McLuckey mechanism, the complete sequence of 8-14-base oligomers and extensive sequence information for a 21-mer and a 25-mer. The spectrum from collisionally activated dissociation of an 8-mer is closely similar to its NS spectrum. Several spectra per minute can be measured, predicting a key role for ESI/MS in nucleotide sequence verification.

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Rapid Sequencing of Oligonucleotides by High-Resolution Mass Spectrometry

Abstract

ASJC Scopus subject areas

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