Rapid transport of neural intermediate filament protein

Brian T. Helfand, Patty Loomis, Miri Yoon, Robert D. Goldman*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

58 Scopus citations

Abstract

Peripherin is a neural intermediate filament protein that is expressed in peripheral and enteric neurons, as well as in PC12 cells. A determination of the motile properties of peripherin has been undertaken in PC12 cells during different stages of neurite outgrowth. The results reveal that non-filamentous, non-membrane bound peripherin particles and short peripherin intermediate filaments, termed 'squiggles', are transported at high speed throughout PC12 cell bodies, neurites and growth cones. These movements are bi-directional, and the majority require microtubules along with their associated molecular motors, conventional kinesin and cytoplasmic dynein. Our data demonstrate that peripherin particles and squiggles can move as components of a rapid transport system capable of delivering cytoskeletal subunits to the most distal regions of neurites over relatively short time periods.

Original languageEnglish (US)
Pages (from-to)2345-2359
Number of pages15
JournalJournal of cell science
Volume116
Issue number11
DOIs
StatePublished - Jun 1 2003

Keywords

  • Cytoskeleton
  • Dynein
  • Intermediate filaments
  • Kinesin
  • Peripherin

ASJC Scopus subject areas

  • Cell Biology

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