RAS mutations in cutaneous squamous-cell carcinomas in patients treated with BRAF inhibitors

Fei Su, Amaya Viros, Carla Milagre, Kerstin Trunzer, Gideon Bollag, Olivia Spleiss, Jorge S. Reis-Filho, Xiangju Kong, Richard C. Koya, Keith T. Flaherty, Paul B. Chapman, Min Jung Kim, Robert Hayward, Matthew Martin, Hong Yang, Qiongqing Wang, Holly Hilton, Julie S. Hang, Johannes Noe, Maryou LambrosFelipe Geyer, Nathalie Dhomen, Ion Niculescu-Duvaz, Alfonso Zambon, Dan Niculescu-Duvaz, Natasha Preece, Lídia Robert, Nicholas J. Otte, Stephen Mok, Damien Kee, Yan Ma, Chao Zhang, Gaston Habets, Elizabeth A. Burton, Bernice Wong, Hoa Nguyen, Mark Kockx, Luc Andries, Brian Lestini, Keith B. Nolop, Richard J. Lee, Andrew K. Joe, James L. Troy, Rene Gonzalez, Thomas E. Hutson, Igor Puzanov, Bartosz Chmielowski, Caroline J. Springer, Grant A. McArthur, Jeffrey A. Sosman, Roger S. Lo, Antoni Ribas*, Richard Marais

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

797 Scopus citations

Abstract

BACKGROUND: Cutaneous squamous-cell carcinomas and keratoacanthomas are common findings in patients treated with BRAF inhibitors. METHODS: We performed a molecular analysis to identify oncogenic mutations (HRAS, KRAS, NRAS, CDKN2A, and TP53) in the lesions from patients treated with the BRAF inhibitor vemurafenib. An analysis of an independent validation set and functional studies with BRAF inhibitors in the presence of the prevalent RAS mutation was also performed. RESULTS: Among 21 tumor samples, 13 had RAS mutations (12 in HRAS). In a validation set of 14 samples, 8 had RAS mutations (4 in HRAS). Thus, 60% (21 of 35) of the specimens harbored RAS mutations, the most prevalent being HRAS Q61L. Increased proliferation of HRAS Q61L-mutant cell lines exposed to vemurafenib was associated with mitogen-activated protein kinase (MAPK)-pathway signaling and activation of ERK-mediated transcription. In a mouse model of HRAS Q61L-mediated skin carcinogenesis, the vemurafenib analogue PLX4720 was not an initiator or a promoter of carcinogenesis but accelerated growth of the lesions harboring HRAS mutations, and this growth was blocked by concomitant treatment with a MEK inhibitor. CONCLUSIONS: Mutations in RAS, particularly HRAS, are frequent in cutaneous squamous-cell carcinomas and keratoacanthomas that develop in patients treated with vemurafenib. The molecular mechanism is consistent with the paradoxical activation of MAPK signaling and leads to accelerated growth of these lesions. (Funded by Hoffmann-La Roche and others; ClinicalTrials.gov numbers, NCT00405587, NCT00949702, NCT01001299, and NCT01006980.)

Original languageEnglish (US)
Pages (from-to)207-215
Number of pages9
JournalNew England Journal of Medicine
Volume366
Issue number3
DOIs
StatePublished - Jan 19 2012

ASJC Scopus subject areas

  • Medicine(all)

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